Java tutorial
/* The MIT License (MIT) Copyright (c) 2014 Pierre Lindenbaum Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. History: * 2015 : creation */ package com.github.lindenb.jvarkit.tools.misc; import java.util.Collection; import java.util.Collections; import java.util.LinkedList; import htsjdk.samtools.SamReader; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMFileWriter; import htsjdk.samtools.SAMRecord; import htsjdk.samtools.SAMRecordIterator; import htsjdk.samtools.util.CloserUtil; import com.github.lindenb.jvarkit.util.picard.SAMSequenceDictionaryProgress; public class BamTile extends AbstractBamTile { private static final org.apache.commons.logging.Log LOG = org.apache.commons.logging.LogFactory .getLog(BamTile.class); public BamTile() { } @Override public Collection<Throwable> call(final String inputName) throws Exception { SAMRecordIterator iter = null; SamReader sfr = null; SAMFileWriter sfw = null; try { sfr = openSamReader(inputName); SAMFileHeader header1 = sfr.getFileHeader(); if (header1 == null) { return wrapException("File header missing"); } if (header1.getSortOrder() != SAMFileHeader.SortOrder.coordinate) { return wrapException("File header not sorted on coordinate"); } SAMFileHeader header2 = header1.clone(); header2.addComment(getName() + ":" + getVersion() + ":" + getProgramCommandLine()); sfw = openSAMFileWriter(header2, true); SAMSequenceDictionaryProgress progress = new SAMSequenceDictionaryProgress(header1); iter = sfr.iterator(); LinkedList<SAMRecord> buffer = new LinkedList<>(); for (;;) { SAMRecord rec = null; if (iter.hasNext()) { rec = progress.watch(iter.next()); if (rec.getReadUnmappedFlag()) continue; if (!buffer.isEmpty()) { SAMRecord last = buffer.getLast(); if (last.getReferenceIndex() == rec.getReferenceIndex() && last.getAlignmentStart() <= rec.getAlignmentStart() && last.getAlignmentEnd() >= rec.getAlignmentEnd()) { continue; } } } if (rec == null || (!buffer.isEmpty() && buffer.getLast().getReferenceIndex() != rec.getReferenceIndex())) { while (!buffer.isEmpty()) { sfw.addAlignment(buffer.removeFirst()); } if (rec == null) break; } buffer.add(rec); if (buffer.size() > 2) { int index = buffer.size(); SAMRecord prev = buffer.get(index - 3); SAMRecord curr = buffer.get(index - 2); SAMRecord next = buffer.get(index - 1); if (prev.getAlignmentEnd() >= next.getAlignmentStart() || curr.getAlignmentEnd() <= prev.getAlignmentEnd()) { buffer.remove(index - 2); } else if (curr.getAlignmentStart() == prev.getAlignmentStart() && curr.getAlignmentEnd() > prev.getAlignmentEnd()) { buffer.remove(index - 3); } } while (buffer.size() > 3) { sfw.addAlignment(buffer.removeFirst()); } } progress.finish(); LOG.info("done"); return Collections.emptyList(); } catch (Exception err) { return wrapException(err); } finally { CloserUtil.close(iter); CloserUtil.close(sfr); CloserUtil.close(sfw); } } /** * @param args */ public static void main(String[] args) { new BamTile().instanceMainWithExit(args); } }