List of usage examples for javax.swing UIManager getSystemLookAndFeelClassName
public static String getSystemLookAndFeelClassName()
LookAndFeel class that implements the native system look and feel if there is one, otherwise the name of the default cross platform LookAndFeel class. From source file:com.adito.server.Main.java
public Integer start(String[] args) { startupStarted = System.currentTimeMillis(); // Inform the wrapper the startup process may take a while if (useWrapper) { WrapperManager.signalStarting(60000); }// w w w . j av a 2 s .c om // Parse the command line Integer returnCode = parseCommandLine(args); if (returnCode != null) { if (returnCode.intValue() == 999) { return null; } return returnCode; } // Create the boot progress monitor if (gui) { try { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (Exception e) { } bootProgressMonitor = new SwingBootProgressMonitor(); } else { bootProgressMonitor = new LogBootProgressMonitor(); } // resourceCaches = new HashMap<URL, ResourceCache>(); contextListeners = new ArrayList<ContextListener>(); loadSystemProperties(); initialiseLogging(); /* * Migrate preferences. */ File newPrefDir = new File(ContextHolder.getContext().getConfDirectory(), "prefs"); PREF = PropertyPreferences.SYSTEM_ROOT; try { if (!newPrefDir.exists() && Preferences.systemRoot().node("/com").nodeExists("adito")) { Preferences from = Preferences.systemRoot().node("/com/adito"); log.warn("Migrating preferences"); try { copyNode(from.node("core"), PREF.node("core")); from.node("core").removeNode(); copyNode(from.node("plugin"), PREF.node("plugin")); from.node("plugin").removeNode(); copyNode(from.node("extensions"), PREF.node("extensions")); from.node("extensions").removeNode(); copyNode(from.node("dbupgrader"), PREF.node("dbupgrader")); from.node("dbupgrader").removeNode(); } catch (Exception e) { log.error("Failed to migrate preferences.", e); } try { from.flush(); } catch (BackingStoreException bse) { log.error("Failed to flush old preferences"); } try { PREF.flush(); } catch (BackingStoreException bse) { log.error("Failed to flush new preferences"); } if (log.isInfoEnabled()) { log.info("Flushing preferences"); } } } catch (BackingStoreException bse) { log.error("Failed to migrate preferences.", bse); } // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } try { clearTemp(); try { hostname = Inet4Address.getLocalHost().getCanonicalHostName(); hostAddress = Inet4Address.getLocalHost().getHostAddress(); } catch (Exception ex) { // This should be fatal, we now rely on the hostname being // available throw new Exception("The host name or address on which this service is running could not " + "be determined. Check you network configuration. One possible cause is " + "a misconfigured 'hosts' file (e.g. on UNIX-like systems this would be " + "/etc/hosts, on Windows XP it would be " + "C:\\Windows\\System32\\Drivers\\Etc\\Hosts)."); } PropertyClassManager.getInstance() .registerPropertyClass(contextConfiguration = new ContextConfig(getClass().getClassLoader())); // Display some information about the system we are running on displaySystemInfo(); // Load the context property definitions loadContextProperties(); // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } // Configure any HTTP / HTTPS / SOCKS proxy servers configureProxyServers(); PropertyList l = contextConfiguration.retrievePropertyList(new ContextKey("webServer.bindAddress")); getBootProgressMonitor().updateMessage("Creating server lock"); getBootProgressMonitor().updateProgress(6); serverLock = new ServerLock((String) l.get(0)); if (serverLock.isLocked()) { if (!isSetupMode()) { if (serverLock.isSetup()) { throw new Exception("The installation wizard is currently running. " + "Please shut this down by pointing your browser " + "to http://" + getHostname() + ":" + serverLock.getPort() + "/showShutdown.do before attempting to start the server again."); } else { throw new Exception("The server is already running."); } } else { if (!serverLock.isSetup()) { throw new Exception("The server is currently already running. " + "Please shut this down by pointing your browser " + "to https://" + getHostname() + ":" + serverLock.getPort() + "/showShutdown.do before attempting to start the server again."); } else { throw new Exception("The installation wizard is running.."); } } } // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } Runtime.getRuntime().addShutdownHook(new Thread() { public void run() { serverLock.stop(); } }); // registerKeyStores(); // threadGroup = new ThreadGroup("MainThreadGroup"); if (install) { setupMode(); } else { normalMode(); startHttpServer(); } } catch (Throwable t) { startupException = t; log.error("Failed to start the server. " + t.getMessage(), t); return new Integer(1); } return null; }
From source file:net.sf.nmedit.nomad.core.NomadLoader.java
private void initLookAndFeel(String lafClassName, String themeClassName, String defaultLafOnPlatform) { EnumSet<Platform.OS> defaultLafPlatforms = EnumSet.noneOf(Platform.OS.class); {//w ww . j av a2 s . c o m // remove whitespace + lowercase defaultLafOnPlatform = defaultLafOnPlatform.replaceAll("\\s", "").toLowerCase(); // split comma separated list String[] dlop = defaultLafOnPlatform.split(","); // check items for (String s : dlop) { if (s.equals("all")) { // on all platforms defaultLafPlatforms.addAll(EnumSet.allOf(Platform.OS.class)); break; } else if (s.equals("mac")) { defaultLafPlatforms.add(Platform.OS.MacOSFlavor); } else if (s.equals("unix")) { defaultLafPlatforms.add(Platform.OS.UnixFlavor); } else if (s.equals("windows")) { defaultLafPlatforms.add(Platform.OS.WindowsFlavor); } } } // jgoodies specific properties PlasticLookAndFeel.setTabStyle(PlasticLookAndFeel.TAB_STYLE_METAL_VALUE); //UIManager.put(Options.POPUP_DROP_SHADOW_ENABLED_KEY, Boolean.FALSE); Options.setPopupDropShadowEnabled(false); Options.setUseNarrowButtons(true); //UIManager.put(Options.PLASTIC_MENU_FONT_KEY, new FontUIResource("Verdana", Font.PLAIN, 9)); //PlasticLookAndFeel.setFontPolicy(FontPolicies.getDefaultWindowsPolicy()); /* UIManager.put("MenuItem.margin", new InsetsUIResource(2,2,1,2)); UIManager.put("Menu.margin", new InsetsUIResource(1,2,1,2)); */ // set the metal theme if (defaultLafPlatforms.contains(Platform.flavor())) { // use default LAF on current platform try { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (Throwable e) { Log log = LogFactory.getLog(getClass()); log.warn("could not set look and feel theme", e); } if (Platform.isFlavor(Platform.OS.MacOSFlavor)) { System.setProperty("apple.laf.useScreenMenuBar", "true"); } } else { // use LAF setting MetalTheme theme = null; if (themeClassName != null) { try { theme = (MetalTheme) Class.forName(themeClassName).newInstance(); UIManager.put("Plastic.theme", themeClassName); if (theme instanceof PlasticTheme) { PlasticLookAndFeel.setPlasticTheme((PlasticTheme) theme); // PlasticLookAndFeel.setTabStyle(settings.getPlasticTabStyle()); } else if (theme instanceof MetalTheme) { MetalLookAndFeel.setCurrentTheme(theme); } } catch (Throwable e) { Log log = LogFactory.getLog(getClass()); log.warn("could not set look and feel theme", e); } } // set the look and feel if (lafClassName != null) { try { LookAndFeel LAF = (LookAndFeel) Class.forName(lafClassName).newInstance(); // it is very important to set the classloader UIManager.getDefaults().put("ClassLoader", getClass().getClassLoader()); UIManager.setLookAndFeel(LAF); } catch (Throwable e) { Log log = LogFactory.getLog(getClass()); log.warn("could not set custom look and feel", e); } } } }
From source file:be.ac.ua.comp.scarletnebula.gui.windows.GUI.java
private void chooseLookAndFeel() { try {//from ww w . j a v a 2 s . c o m boolean found = false; for (final LookAndFeelInfo info : UIManager.getInstalledLookAndFeels()) { if ("Nimbus".equals(info.getName())) { UIManager.setLookAndFeel(info.getClassName()); found = true; break; } } if (!found) { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } } catch (final Exception e) { log.error("Cannot set look and feel", e); } }
From source file:com.sslexplorer.server.Main.java
public Integer start(String[] args) { startupStarted = System.currentTimeMillis(); // Inform the wrapper the startup process may take a while if (useWrapper) { WrapperManager.signalStarting(60000); }/* www.j av a 2s .c o m*/ // Parse the command line Integer returnCode = parseCommandLine(args); if (returnCode != null) { if (returnCode.intValue() == 999) { return null; } return returnCode; } // Create the boot progress monitor if (gui) { try { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (Exception e) { } bootProgressMonitor = new SwingBootProgressMonitor(); } else { bootProgressMonitor = new LogBootProgressMonitor(); } // resourceCaches = new HashMap<URL, ResourceCache>(); contextListeners = new ArrayList<ContextListener>(); loadSystemProperties(); initialiseLogging(); /* * Migrate preferences. */ File newPrefDir = new File(ContextHolder.getContext().getConfDirectory(), "prefs"); PREF = PropertyPreferences.SYSTEM_ROOT; try { if (!newPrefDir.exists() && Preferences.systemRoot().node("/com").nodeExists("sslexplorer")) { Preferences from = Preferences.systemRoot().node("/com/sslexplorer"); log.warn("Migrating preferences"); try { copyNode(from.node("core"), PREF.node("core")); from.node("core").removeNode(); copyNode(from.node("plugin"), PREF.node("plugin")); from.node("plugin").removeNode(); copyNode(from.node("extensions"), PREF.node("extensions")); from.node("extensions").removeNode(); copyNode(from.node("dbupgrader"), PREF.node("dbupgrader")); from.node("dbupgrader").removeNode(); } catch (Exception e) { log.error("Failed to migrate preferences.", e); } try { from.flush(); } catch (BackingStoreException bse) { log.error("Failed to flush old preferences"); } try { PREF.flush(); } catch (BackingStoreException bse) { log.error("Failed to flush new preferences"); } if (log.isInfoEnabled()) { log.info("Flushing preferences"); } } } catch (BackingStoreException bse) { log.error("Failed to migrate preferences.", bse); } // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } try { clearTemp(); try { hostname = Inet4Address.getLocalHost().getCanonicalHostName(); hostAddress = Inet4Address.getLocalHost().getHostAddress(); } catch (Exception ex) { // This should be fatal, we now rely on the hostname being // available throw new Exception("The host name or address on which this service is running could not " + "be determined. Check you network configuration. One possible cause is " + "a misconfigured 'hosts' file (e.g. on UNIX-like systems this would be " + "/etc/hosts, on Windows XP it would be " + "C:\\Windows\\System32\\Drivers\\Etc\\Hosts)."); } PropertyClassManager.getInstance() .registerPropertyClass(contextConfiguration = new ContextConfig(getClass().getClassLoader())); // Display some information about the system we are running on displaySystemInfo(); // Load the context property definitions loadContextProperties(); // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } // Configure any HTTP / HTTPS / SOCKS proxy servers configureProxyServers(); PropertyList l = contextConfiguration.retrievePropertyList(new ContextKey("webServer.bindAddress")); getBootProgressMonitor().updateMessage("Creating server lock"); getBootProgressMonitor().updateProgress(6); serverLock = new ServerLock((String) l.get(0)); if (serverLock.isLocked()) { if (!isSetupMode()) { if (serverLock.isSetup()) { throw new Exception("The installation wizard is currently running. " + "Please shut this down by pointing your browser " + "to http://" + getHostname() + ":" + serverLock.getPort() + "/showShutdown.do before attempting to start the server again."); } else { throw new Exception("The server is already running."); } } else { if (!serverLock.isSetup()) { throw new Exception("The server is currently already running. " + "Please shut this down by pointing your browser " + "to https://" + getHostname() + ":" + serverLock.getPort() + "/showShutdown.do before attempting to start the server again."); } else { throw new Exception("The installation wizard is running.."); } } } // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } Runtime.getRuntime().addShutdownHook(new Thread() { public void run() { serverLock.stop(); } }); // registerKeyStores(); // threadGroup = new ThreadGroup("MainThreadGroup"); if (install) { setupMode(); } else { normalMode(); startHttpServer(); } } catch (Throwable t) { startupException = t; log.error("Failed to start the server. " + t.getMessage(), t); return new Integer(1); } return null; }
From source file:com.enderville.enderinstaller.ui.Installer.java
public static void main(String[] args) throws IOException { LOGGER.debug("Starting EnderPack Installer"); LOGGER.debug("Current OS: " + InstallerConfig.currentOS); if (!sanityCheck()) { return;/*from w w w. ja v a2s . co m*/ } try { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (Exception e) { LOGGER.warn("Couldn't set the look and feel", e); } Installer installer = new Installer(); installer.setVisible(true); }
From source file:com.adito.server.DefaultAditoServerFactory.java
public Integer start(String[] args) { startupStarted = System.currentTimeMillis(); // Inform the wrapper the startup process may take a while if (useWrapper) { WrapperManager.signalStarting(60000); }/*from w w w . j ava 2s . c om*/ // Parse the command line Integer returnCode = parseCommandLine(args); if (returnCode != null) { if (returnCode == 999) { return null; } return returnCode; } // Create the boot progress monitor if (gui) { try { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (ClassNotFoundException e) { } catch (InstantiationException e) { } catch (IllegalAccessException e) { } catch (UnsupportedLookAndFeelException e) { } bootProgressMonitor = new SwingBootProgressMonitor(); } else { bootProgressMonitor = new LogBootProgressMonitor(); } // resourceCaches = new HashMap<URL, ResourceCache>(); contextListeners = new ArrayList<ContextListener>(); loadSystemProperties(); initialiseLogging(); /* * Migrate preferences. */ File newPrefDir = new File(ContextHolder.getContext().getConfDirectory(), "prefs"); PREF = PropertyPreferences.SYSTEM_ROOT; try { if (!newPrefDir.exists() && Preferences.systemRoot().node("/com").nodeExists("adito")) { Preferences from = Preferences.systemRoot().node("/com/adito"); LOG.warn("Migrating preferences"); try { copyNode(from.node("core"), PREF.node("core")); from.node("core").removeNode(); copyNode(from.node("plugin"), PREF.node("plugin")); from.node("plugin").removeNode(); copyNode(from.node("extensions"), PREF.node("extensions")); from.node("extensions").removeNode(); copyNode(from.node("dbupgrader"), PREF.node("dbupgrader")); from.node("dbupgrader").removeNode(); } catch (BackingStoreException e) { LOG.error("Failed to migrate preferences.", e); } try { from.flush(); } catch (BackingStoreException bse) { LOG.error("Failed to flush old preferences"); } try { PREF.flush(); } catch (BackingStoreException bse) { LOG.error("Failed to flush new preferences"); } if (LOG.isInfoEnabled()) { LOG.info("Flushing preferences"); } } } catch (BackingStoreException bse) { LOG.error("Failed to migrate preferences.", bse); } // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } try { clearTemp(); try { hostname = Inet4Address.getLocalHost().getCanonicalHostName(); hostAddress = Inet4Address.getLocalHost().getHostAddress(); } catch (UnknownHostException ex) { // This should be fatal, we now rely on the hostname being // available throw new Exception("The host name or address on which this service is running could not " + "be determined. Check you network configuration. One possible cause is " + "a misconfigured 'hosts' file (e.g. on UNIX-like systems this would be " + "/etc/hosts, on Windows XP it would be " + "C:\\Windows\\System32\\Drivers\\Etc\\Hosts)."); } PropertyClassManager.getInstance() .registerPropertyClass(contextConfiguration = new ContextConfig(getClass().getClassLoader())); // Display some information about the system we are running on displaySystemInfo(); // Load the context property definitions loadContextProperties(); // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } // Configure any HTTP / HTTPS / SOCKS proxy servers configureProxyServers(); PropertyList l = contextConfiguration.retrievePropertyList(new ContextKey("webServer.bindAddress")); getBootProgressMonitor().updateMessage("Creating server lock"); getBootProgressMonitor().updateProgress(6); serverLock = new ServerLock(l.get(0)); if (serverLock.isLocked()) { if (!isSetupMode()) { if (serverLock.isSetup()) { throw new Exception("The installation wizard is currently running. " + "Please shut this down by pointing your browser " + "to http://" + getHostname() + ":" + serverLock.getPort() + "/showShutdown.do before attempting to start the server again."); } else { throw new Exception("The server is already running."); } } else { if (!serverLock.isSetup()) { throw new Exception("The server is currently already running. " + "Please shut this down by pointing your browser " + "to https://" + getHostname() + ":" + serverLock.getPort() + "/showShutdown.do before attempting to start the server again."); } else { throw new Exception("The installation wizard is running.."); } } } // Inform the wrapper the startup process is going ok if (useWrapper) { WrapperManager.signalStarting(60000); } Runtime.getRuntime().addShutdownHook(new Thread() { @Override public void run() { serverLock.stop(); } }); // registerKeyStores(); // threadGroup = new ThreadGroup("MainThreadGroup"); if (install) { setupMode(); } else { normalMode(); startHttpServer(); } } catch (Exception t) { startupException = t; LOG.error("Failed to start the server. " + t.getMessage(), t); return 1; } return null; }
From source file:strobe.spectroscopy.StrobeSpectroscopy.java
/** * @param args the command line arguments *//* w ww. j a v a 2 s. co m*/ public static void main(String args[]) { /* Set the Nimbus look and feel */ //<editor-fold defaultstate="collapsed" desc=" Look and feel setting code (optional) "> /* If Nimbus (introduced in Java SE 6) is not available, stay with the default look and feel. * For details see http://download.oracle.com/javase/tutorial/uiswing/lookandfeel/plaf.html */ try { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (ClassNotFoundException ex) { java.util.logging.Logger.getLogger(StrobeSpectroscopy.class.getName()) .log(java.util.logging.Level.SEVERE, null, ex); } catch (InstantiationException ex) { java.util.logging.Logger.getLogger(StrobeSpectroscopy.class.getName()) .log(java.util.logging.Level.SEVERE, null, ex); } catch (IllegalAccessException ex) { java.util.logging.Logger.getLogger(StrobeSpectroscopy.class.getName()) .log(java.util.logging.Level.SEVERE, null, ex); } catch (javax.swing.UnsupportedLookAndFeelException ex) { java.util.logging.Logger.getLogger(StrobeSpectroscopy.class.getName()) .log(java.util.logging.Level.SEVERE, null, ex); } //</editor-fold> /* Create and display the form */ java.awt.EventQueue.invokeLater(new Runnable() { public void run() { new StrobeSpectroscopy().setVisible(true); } }); }
From source file:com.unicorn.co226.ui.patient.AddStudentForm.java
/** * @param args the command line arguments */// w w w .j a va2 s .co m public static void main(String args[]) { try { /* Set the Nimbus look and feel */ // //<editor-fold defaultstate="collapsed" desc=" Look and feel setting code (optional) "> // /* If Nimbus (introduced in Java SE 6) is not available, stay with the default look and feel. // * For details see http://download.oracle.com/javase/tutorial/uiswing/lookandfeel/plaf.html // */ // try { // for (javax.swing.UIManager.LookAndFeelInfo info : javax.swing.UIManager.getInstalledLookAndFeels()) { // if ("Nimbus".equals(info.getName())) { // javax.swing.UIManager.setLookAndFeel(info.getClassName()); // break; // } // } // } catch (ClassNotFoundException ex) { // java.util.logging.Logger.getLogger(AddStudentForm.class.getName()).log(java.util.logging.Level.SEVERE, null, ex); // } catch (InstantiationException ex) { // java.util.logging.Logger.getLogger(AddStudentForm.class.getName()).log(java.util.logging.Level.SEVERE, null, ex); // } catch (IllegalAccessException ex) { // java.util.logging.Logger.getLogger(AddStudentForm.class.getName()).log(java.util.logging.Level.SEVERE, null, ex); // } catch (javax.swing.UnsupportedLookAndFeelException ex) { // java.util.logging.Logger.getLogger(AddStudentForm.class.getName()).log(java.util.logging.Level.SEVERE, null, ex); // } // //</editor-fold> UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (ClassNotFoundException ex) { Logger.getLogger(AddStudentForm.class.getName()).log(Level.SEVERE, null, ex); } catch (InstantiationException ex) { Logger.getLogger(AddStudentForm.class.getName()).log(Level.SEVERE, null, ex); } catch (IllegalAccessException ex) { Logger.getLogger(AddStudentForm.class.getName()).log(Level.SEVERE, null, ex); } catch (UnsupportedLookAndFeelException ex) { Logger.getLogger(AddStudentForm.class.getName()).log(Level.SEVERE, null, ex); } /* Create and display the dialog */ java.awt.EventQueue.invokeLater(new Runnable() { public void run() { AddStudentForm dialog = new AddStudentForm(new javax.swing.JFrame(), true); dialog.addWindowListener(new java.awt.event.WindowAdapter() { @Override public void windowClosing(java.awt.event.WindowEvent e) { System.exit(0); } }); dialog.setVisible(true); } }); }
From source file:com.diversityarrays.kdxplore.KDXplore.java
public static void initLAF() { // GuiUtil.initLookAndFeel(); try {//from w w w. jav a2s .c o m for (UIManager.LookAndFeelInfo lafi : UIManager.getInstalledLookAndFeels()) { System.out.println(lafi.getName() + "\t" + lafi.getClassName()); //$NON-NLS-1$ } System.out.println("-----"); //$NON-NLS-1$ // String slaf = "javax.swing.plaf.nimbus.NimbusLookAndFeel"; // // UIManager.getSystemLookAndFeelClassName(); // String slaf = "com.sun.java.swing.plaf.motif.MotifLookAndFeel"; // // UIManager.getSystemLookAndFeelClassName(); // String slaf = "javax.swing.plaf.metal.MetalLookAndFeel"; // // UIManager.getSystemLookAndFeelClassName(); String slaf = UIManager.getSystemLookAndFeelClassName(); UIManager.setLookAndFeel(slaf); } catch (ClassNotFoundException e) { e.printStackTrace(); } catch (InstantiationException e) { e.printStackTrace(); } catch (IllegalAccessException e) { e.printStackTrace(); } catch (UnsupportedLookAndFeelException e) { e.printStackTrace(); } finally { } }
From source file:MiGA.StatsSelection.java
public StatsSelection(final String[] organisms, final boolean flag) { setTitle("MiGA"); setSize(800, 600);//from www . ja v a 2 s. c o m setLocationRelativeTo(null); setResizable(false); setDefaultCloseOperation(JFrame.EXIT_ON_CLOSE); Image im = Toolkit.getDefaultToolkit().getImage("ssr.png"); this.setIconImage(im); try { UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName()); } catch (Exception e) { } countmono = new ArrayList<Integer>(); countdi = new ArrayList<Integer>(); counttri = new ArrayList<Integer>(); counttetra = new ArrayList<Integer>(); countpenta = new ArrayList<Integer>(); counthexa = new ArrayList<Integer>(); countmonore = new ArrayList<Integer>(); countdire = new ArrayList<Integer>(); counttrire = new ArrayList<Integer>(); counttetrare = new ArrayList<Integer>(); countpentare = new ArrayList<Integer>(); counthexare = new ArrayList<Integer>(); Amono = new ArrayList<Integer>(); Tmono = new ArrayList<Integer>(); Gmono = new ArrayList<Integer>(); Cmono = new ArrayList<Integer>(); Adi = new ArrayList<Integer>(); Tdi = new ArrayList<Integer>(); Gdi = new ArrayList<Integer>(); Cdi = new ArrayList<Integer>(); Atri = new ArrayList<Integer>(); Ttri = new ArrayList<Integer>(); Gtri = new ArrayList<Integer>(); Ctri = new ArrayList<Integer>(); Atetra = new ArrayList<Integer>(); Ttetra = new ArrayList<Integer>(); Gtetra = new ArrayList<Integer>(); Ctetra = new ArrayList<Integer>(); Apenta = new ArrayList<Integer>(); Tpenta = new ArrayList<Integer>(); Gpenta = new ArrayList<Integer>(); Cpenta = new ArrayList<Integer>(); Ahexa = new ArrayList<Integer>(); Thexa = new ArrayList<Integer>(); Ghexa = new ArrayList<Integer>(); Chexa = new ArrayList<Integer>(); for (int i = 0; i < organisms.length; i++) { countmono.add(0); countdi.add(0); counttri.add(0); counttetra.add(0); countpenta.add(0); counthexa.add(0); countmonore.add(0); countdire.add(0); counttrire.add(0); counttetrare.add(0); countpentare.add(0); counthexare.add(0); Amono.add(0); Tmono.add(0); Gmono.add(0); Cmono.add(0); Adi.add(0); Tdi.add(0); Gdi.add(0); Cdi.add(0); Atri.add(0); Ttri.add(0); Gtri.add(0); Ctri.add(0); Atetra.add(0); Ttetra.add(0); Gtetra.add(0); Ctetra.add(0); Apenta.add(0); Tpenta.add(0); Gpenta.add(0); Cpenta.add(0); Ahexa.add(0); Thexa.add(0); Ghexa.add(0); Chexa.add(0); } lab = new JLabel( "<html><b><p>To retrieve the sequence you want</p><p>simply copy and paste in the fields below</p><p>the data you were given in your result's file</p></b></html>"); startlab = new JLabel("Start:"); endlab = new JLabel("End:"); titlelab = new JLabel("Chromosome or field:"); startnum = new JTextField(); startnum.setColumns(5); endnum = new JTextField(); endnum.setColumns(5); titlef = new JTextField(); titlef.setColumns(30); flankst = new JLabel("Flanking region before: "); flankst.setEnabled(false); flankst.setVisible(false); flankend = new JLabel("Flanking region after: "); flankend.setEnabled(false); flankend.setVisible(false); flankstn = new JTextField(); flankstn.setColumns(5); flankstn.setEnabled(false); flankstn.setVisible(false); flankendn = new JTextField(); flankendn.setColumns(5); flankendn.setEnabled(false); flankendn.setVisible(false); result = new JTextArea("", 6, 90); result.setText(" "); result.setEditable(false); result.setLineWrap(true); result.setAutoscrolls(true); sbrText = new JScrollPane(result); sbrText.setVerticalScrollBarPolicy(JScrollPane.VERTICAL_SCROLLBAR_ALWAYS); flk = new JCheckBox("Flanking Regions"); flk.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { if (flk.isSelected()) { flankst.setEnabled(true); flankst.setVisible(true); flankend.setEnabled(true); flankend.setVisible(true); flankstn.setEnabled(true); flankstn.setVisible(true); flankendn.setEnabled(true); flankendn.setVisible(true); } if (!flk.isSelected()) { flankst.setEnabled(false); flankst.setVisible(false); flankend.setEnabled(false); flankend.setVisible(false); flankstn.setEnabled(false); flankstn.setVisible(false); flankendn.setEnabled(false); flankendn.setVisible(false); } } }); retrieve = new JButton("Retrieve"); retrieve.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { int start = 0; int end = 0; BufferedReader in = null; String location = ""; String newlocation = ""; String bufferpre = ""; String bufferpost = ""; String pre = ""; String post = ""; String mid = ""; try { String[] locationarray = titlef.getText().split("data/"); location = locationarray[0] + locationarray[1]; newlocation = ""; if (location.contains("local")) { in = new BufferedReader(new FileReader(location + ".txt")); } else if (location.contains("organisms")) { String[] loc = location.split("/"); try { if (CheckForKaryotype(loc[1])) { newlocation = loc[0] + "/" + loc[1] + "/chrom-" + loc[2] + "-slices.txt"; } else { newlocation = loc[0] + "/" + loc[1] + "/slice-" + loc[2] + ".txt"; } } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (ClassNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } in = new BufferedReader(new FileReader(newlocation)); } } catch (FileNotFoundException ex) { msg.showMessageDialog(paneldown, "Wrong field", "Error", JOptionPane.ERROR_MESSAGE); } int rest = Integer.parseInt(startnum.getText()) % 20000; int lines = Integer.parseInt(startnum.getText()) / 20000; lines++; String buffer1 = ""; for (int c = 0; c < lines; c++) { try { buffer1 = in.readLine(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } try { in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } if (flk.isSelected()) { boolean tfs = false, tfe = false; int fs = 0, fe = 0; try { fs = Integer.parseInt(flankstn.getText()); tfs = true; } catch (NumberFormatException ex) { tfs = false; } try { fe = Integer.parseInt(flankendn.getText()); tfe = true; } catch (NumberFormatException ex) { tfe = false; } if (tfs && tfe) { start = rest - Integer.parseInt(flankstn.getText()); end = rest + Integer.parseInt(endnum.getText()) - Integer.parseInt(startnum.getText()) + Integer.parseInt(flankendn.getText()); try { in = new BufferedReader(new FileReader(newlocation)); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } if (start < 0 && lines == 1) { start = 0; } else if (start < 0 && lines > 1) { for (int j = 0; j < lines - 1; j++) { try { bufferpre = in.readLine(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } pre = bufferpre.substring(20000 + start); mid = buffer1.substring(0, end); try { in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } int cl = 0; try { cl = countlines(newlocation); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } try { in = new BufferedReader(new FileReader(newlocation)); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } if (end > 20000 && lines == cl - 1) { if (end - 20000 >= rest) { end = rest; mid = buffer1.substring(start); post = bufferpost; } else { for (int j = 0; j < lines - 1; j++) { try { bufferpost = in.readLine(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } mid = buffer1.substring(start); post = bufferpre.substring(0, end - 20000); try { in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } } else if (end > 20000 && lines < cl - 1) { for (int j = 0; j < lines + 1; j++) { try { bufferpost = in.readLine(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } mid = buffer1.substring(start); post = bufferpost.substring(0, end - 20000); try { in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } if (start >= 0 && (end <= 20000 || end <= rest)) { mid = buffer1.substring(start, end); } } else { if (!tfs) { msg.showMessageDialog(paneldown, "Flanking region start is empty.\nFill in the gap or uncheck the\nflanking regions checkbox", "Error", JOptionPane.ERROR_MESSAGE); } if (!tfe) { msg.showMessageDialog(paneldown, "Flanking region end is empty.\nFill in the gap or uncheck the\nflanking regions checkbox", "Error", JOptionPane.ERROR_MESSAGE); } } // } if (!flk.isSelected()) { start = rest; end = rest + Integer.parseInt(endnum.getText()) - Integer.parseInt(startnum.getText()); try { in = new BufferedReader(new FileReader(newlocation)); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } if (start < 0 && lines == 1) { start = 0; } else if (start < 0 && lines > 1) { for (int j = 0; j < lines - 1; j++) { try { bufferpre = in.readLine(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } pre = bufferpre.substring(start); mid = buffer1.substring(0, end); try { in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } int cl = 0; try { cl = countlines(newlocation); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } try { in = new BufferedReader(new FileReader(newlocation)); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } if (end > 20000 && lines == cl - 1) { if (end - 20000 >= rest) { end = rest; mid = buffer1.substring(start); post = bufferpost; } else { for (int j = 0; j < lines - 1; j++) { try { bufferpost = in.readLine(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } mid = buffer1.substring(start); post = bufferpre.substring(0, end - 20000); try { in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } } else if (end > 20000 && lines < cl - 1) { for (int j = 0; j < lines + 1; j++) { try { bufferpost = in.readLine(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } mid = buffer1.substring(start); post = bufferpost.substring(0, end - 20000); try { in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } if (start >= 0 && (end <= 20000 || end <= rest)) { mid = buffer1.substring(start, end); } } result.setText(pre + mid + post); } }); mono = new JCheckBox("Mononucleotide"); di = new JCheckBox("Dinucleotide"); tri = new JCheckBox("Trinucleotide"); tetra = new JCheckBox("Tetranucleotide"); penta = new JCheckBox("Pentanucleotide"); hexa = new JCheckBox("Hexanucleotide"); SSR = new ArrayList<String>(); repeats = new ArrayList<Integer>(); EndOfSsr = new ArrayList<Integer>(); start = new ArrayList<Integer>(); select = new JLabel("Select type: "); minimumssrlen = new JLabel("Minimum SSR length(bp)"); minimumssrlen.setVisible(false); score = new JTextField(); score.setColumns(5); score.setVisible(false); msg = new JOptionPane(); gapmax = new JLabel("Maximum Mismatch length for Imperfect SSRs(bp)"); gapmax.setVisible(false); max = new JTextField(); max.setColumns(5); max.setVisible(false); minlenpregap = new JLabel("Minimum SSR length before given Mismatch length(bp)"); minlenpregap.setVisible(false); minpregap = new JTextField(); minpregap.setColumns(5); minpregap.setVisible(false); gapcomp = new JLabel("Maximum Inter-repeat R for Compound SSRs(bp)"); gapcomp.setVisible(false); maxgapcomp = new JTextField(); maxgapcomp.setColumns(5); maxgapcomp.setVisible(false); box1 = new JCheckBox("Perfect"); box2 = new JCheckBox("Imperfect"); box3 = new JCheckBox("Compound"); com = new JCheckBox("Perfect Compound"); incom = new JCheckBox("Imperfect Compound"); box1.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { if (box2.isSelected() || box3.isSelected()) { if (box1.isSelected()) { std.setEnabled(true); no_st.setEnabled(true); part_st.setEnabled(true); full_st.setEnabled(true); if (!box3.isSelected()) { minimumssrlen.setVisible(true); score.setVisible(true); } } if (!box1.isSelected()) { std.setEnabled(false); no_st.setEnabled(false); part_st.setEnabled(false); full_st.setEnabled(false); if (!box3.isSelected()) { minimumssrlen.setVisible(false); score.setVisible(false); } } } else { if (box1.isSelected()) { std.setEnabled(true); no_st.setEnabled(true); part_st.setEnabled(true); full_st.setEnabled(true); panel2.setVisible(true); minimumssrlen.setVisible(true); score.setVisible(true); } if (!box1.isSelected()) { std.setEnabled(false); no_st.setEnabled(false); part_st.setEnabled(false); full_st.setEnabled(false); panel2.setVisible(false); minimumssrlen.setVisible(false); score.setVisible(false); } } } }); box2.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { if (box1.isSelected() || box3.isSelected()) { if (box2.isSelected()) { gapmax.setVisible(true); max.setVisible(true); minlenpregap.setVisible(true); minpregap.setVisible(true); } if (!box2.isSelected()) { gapmax.setVisible(false); max.setVisible(false); minlenpregap.setVisible(false); minpregap.setVisible(false); } } else { if (box2.isSelected()) { panel2.setVisible(true); gapmax.setVisible(true); max.setVisible(true); minlenpregap.setVisible(true); minpregap.setVisible(true); } if (!box2.isSelected()) { panel2.setVisible(false); gapmax.setVisible(false); max.setVisible(false); minlenpregap.setVisible(false); minpregap.setVisible(false); } } } }); box3.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { if (box1.isSelected() || box2.isSelected()) { if (box3.isSelected()) { paneldownleft.setVisible(true); if (!box1.isSelected()) { minimumssrlen.setVisible(true); score.setVisible(true); } gapcomp.setVisible(true); maxgapcomp.setVisible(true); com.setVisible(true); incom.setVisible(true); } if (!box3.isSelected()) { paneldownleft.setVisible(false); gapcomp.setVisible(false); maxgapcomp.setVisible(false); if (!box1.isSelected()) { minimumssrlen.setVisible(false); score.setVisible(false); com.setVisible(false); incom.setVisible(false); } } } else { if (box3.isSelected()) { paneldownleft.setVisible(true); panel2.setVisible(true); minimumssrlen.setVisible(true); score.setVisible(true); gapcomp.setVisible(true); maxgapcomp.setVisible(true); com.setVisible(true); incom.setVisible(true); } if (!box3.isSelected()) { paneldownleft.setVisible(false); panel2.setVisible(false); minimumssrlen.setVisible(false); score.setVisible(false); gapcomp.setVisible(false); maxgapcomp.setVisible(false); com.setVisible(false); incom.setVisible(false); } } } }); /* incom.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { if (incom.isSelected()) { if (!box2.isSelected()) { gapmax.setVisible(true); max.setVisible(true); minlenpregap.setVisible(true); minpregap.setVisible(true); } } if (!incom.isSelected()) { if (!box2.isSelected()) { gapmax.setVisible(false); max.setVisible(false); minlenpregap.setVisible(false); minpregap.setVisible(false); } } } });*/ std = new JPanel(); no_st = new JRadioButton("Not Standardized"); part_st = new JRadioButton("Partial Standardized"); full_st = new JRadioButton("Full Standardized"); no_st.setSelected(true); no_st.setEnabled(false); part_st.setEnabled(false); full_st.setEnabled(false); standard = new ButtonGroup(); standard.add(no_st); standard.add(part_st); standard.add(full_st); show = new JButton("Run"); show.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { setCursor(Cursor.getPredefinedCursor(Cursor.WAIT_CURSOR)); if (!box1.isSelected() && !box2.isSelected() && !box3.isSelected()) { msg.showMessageDialog(paneldown, "Please select a type", "Error", JOptionPane.ERROR_MESSAGE); } for (int i = 0; i < organisms.length; i++) { File f = new File("organisms/" + organisms[i] + "/stats/"); if (f.exists()) { f.delete(); } } calendar = Calendar.getInstance(); now = calendar.getTime(); if (box1.isSelected()) { if (!score.getText().isEmpty()) { boolean isnumber = false; int minlen = 0; try { minlen = Integer.parseInt(score.getText()); isnumber = true; } catch (NumberFormatException ex) { isnumber = false; } if (isnumber) { try { getPerfectSSRs(organisms, minlen, flag); for (int i = 0; i < organisms.length; i++) { map = new HashMap<String, motifStats>(); String location = ""; String location2 = ""; PrintWriter out = null; // 18/11/2013 added starting here String filetype = ""; String filepro = ""; if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } //List<String> files = getFiles(organisms[i], minlen, flag); // 18/11/2013 added ending here PrintWriter stats = null; PrintWriter html = null; PrintWriter motifstats = null; PrintWriter motifhtml = null; DataOutputStream lt = null; if (filetype.contains("organisms")) { File f = new File("organisms/" + organisms[i] + "/stats/"); if (!f.exists()) { f.mkdir(); } stats = new PrintWriter(new FileWriter("organisms/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt", true)); motifstats = new PrintWriter(new FileWriter("organisms/" + organisms[i] + "/stats/" + "motif_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt", true)); motifhtml = new PrintWriter(new FileWriter("organisms/" + organisms[i] + "/stats/" + "motif_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".html", true)); html = new PrintWriter(new FileWriter("organisms/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".html", true)); lt = new DataOutputStream(new BufferedOutputStream( new FileOutputStream("organisms/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".perf"))); File fi = new File("organisms/" + organisms[i] + "/results/"); if (!fi.exists()) { fi.mkdir(); } String toopen = "organisms/" + organisms[i] + "/results/allPerfect_" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; location = toopen; location2 = "organisms/" + organisms[i] + "/stats/" + "motif_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; out = new PrintWriter(toopen); out.println("Results for organism: " + organisms[i] + "\t Search Parameters --> Minimum SSR Length (bp): " + minlen); out.println( " SSR repeats start-end length Path(../organism/data/chromosome)"); } else if (filetype.contains("local")) { File f = new File("local/" + organisms[i] + "/stats/"); if (!f.exists()) { f.mkdir(); } stats = new PrintWriter(new FileWriter("local/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt", true)); motifstats = new PrintWriter(new FileWriter("local/" + organisms[i] + "/stats/" + "motif_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt", true)); motifhtml = new PrintWriter(new FileWriter("local/" + organisms[i] + "/stats/" + "motif_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".html", true)); lt = new DataOutputStream(new BufferedOutputStream( new FileOutputStream("local/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".perf"))); html = new PrintWriter(new FileWriter("local/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".html", true)); File fi = new File("local/" + organisms[i] + "/results/"); if (!fi.exists()) { fi.mkdir(); } String toopen = "local/" + organisms[i] + "/results/allPerfect_" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; location = toopen; location2 = "local/" + organisms[i] + "/stats/" + "motif_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; out = new PrintWriter(toopen); out.println("Results for project: " + organisms[i] + "\t Search Parameters --> Minimum SSR Length (bp): " + minlen); out.println( " SSR repeats start-end length Path(../organism/data/chromosome)"); out.println(); } if (mono.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } //List<String> files = getFiles(organisms[i], minlen, flag); //for (int j = 0; j < files.size(); j++) { while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + minlen + "_monoPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int repeats = in.readInt(); int end = in.readInt(); out.println(cell(ssr, 6) + " " + cell(repeats, 11) + " " + cell(Integer .toString(end - repeats * ssr.length() + 1) + "-" + Integer.toString(end + 1), 20) + " " + cell(repeats * ssr.length(), 6) + " " + files.substring(0, files.lastIndexOf('.'))); // map for motifstats if (!map.containsKey(ssr)) { motifStats m = new motifStats(ssr, repeats); map.put(ssr, m); } else { map.get(ssr).update(repeats); } } catch (EOFException exc) { eof = true; } } in.close(); } } if (di.isSelected()) { //for (int j = 0; j < files.size(); j++) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } //List<String> files = getFiles(organisms[i], minlen, flag); while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream(new BufferedInputStream( new FileInputStream(files + "_" + minlen + "_diPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int repeats = in.readInt(); int end = in.readInt(); //out.println("SSR: " + ssr + " repeats: " + repeats + " start-end " + (end - repeats * ssr.length()) + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); out.println(cell(ssr, 6) + " " + cell(repeats, 11) + " " + cell(Integer .toString(end - repeats * ssr.length() + 1) + "-" + Integer.toString(end + 1), 20) + " " + cell(repeats * ssr.length(), 6) + " " + files.substring(0, files.lastIndexOf('.'))); if (!map.containsKey(ssr)) { motifStats m = new motifStats(ssr, repeats); map.put(ssr, m); } else { map.get(ssr).update(repeats); } } catch (EOFException exc) { eof = true; } } in.close(); } } if (tri.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } //for (int j = 0; j < files.size(); j++) { while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + minlen + "_triPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int repeats = in.readInt(); int end = in.readInt(); //out.println("SSR: " + ssr + " repeats: " + repeats + " start-end " + (end - repeats * ssr.length()) + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); out.println(cell(ssr, 6) + " " + cell(repeats, 11) + " " + cell(Integer .toString(end - repeats * ssr.length() + 1) + "-" + Integer.toString(end + 1), 20) + " " + cell(repeats * ssr.length(), 6) + " " + files.substring(0, files.lastIndexOf('.'))); if (!map.containsKey(ssr)) { motifStats m = new motifStats(ssr, repeats); map.put(ssr, m); } else { map.get(ssr).update(repeats); } } catch (EOFException exc) { eof = true; } } in.close(); } } if (tetra.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + minlen + "_tetraPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int repeats = in.readInt(); int end = in.readInt(); // out.println("SSR: " + ssr + " repeats: " + repeats + " start-end " + (end - repeats * ssr.length()) + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); out.println(cell(ssr, 6) + " " + cell(repeats, 11) + " " + cell(Integer .toString(end - repeats * ssr.length() + 1) + "-" + Integer.toString(end + 1), 20) + " " + cell(repeats * ssr.length(), 6) + " " + files.substring(0, files.lastIndexOf('.'))); if (!map.containsKey(ssr)) { motifStats m = new motifStats(ssr, repeats); map.put(ssr, m); } else { map.get(ssr).update(repeats); } } catch (EOFException exc) { eof = true; } } in.close(); } } if (penta.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + minlen + "_pentaPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int repeats = in.readInt(); int end = in.readInt(); // out.println("SSR: " + ssr + " repeats: " + repeats + " start-end " + (end - repeats * ssr.length()) + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); out.println(cell(ssr, 6) + " " + cell(repeats, 11) + " " + cell(Integer .toString(end - repeats * ssr.length() + 1) + "-" + Integer.toString(end + 1), 20) + " " + cell(repeats * ssr.length(), 6) + " " + files.substring(0, files.lastIndexOf('.'))); if (!map.containsKey(ssr)) { motifStats m = new motifStats(ssr, repeats); map.put(ssr, m); } else { map.get(ssr).update(repeats); } } catch (EOFException exc) { eof = true; } } in.close(); } } if (hexa.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + minlen + "_hexaPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int repeats = in.readInt(); int end = in.readInt(); // out.println("SSR: " + ssr + " repeats: " + repeats + " start-end " + (end - repeats * ssr.length()) + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); out.println(cell(ssr, 6) + " " + cell(repeats, 11) + " " + cell(Integer .toString(end - repeats * ssr.length() + 1) + "-" + Integer.toString(end + 1), 20) + " " + cell(repeats * ssr.length(), 6) + " " + files.substring(0, files.lastIndexOf('.'))); if (!map.containsKey(ssr)) { motifStats m = new motifStats(ssr, repeats); map.put(ssr, m); } else { map.get(ssr).update(repeats); } } catch (EOFException exc) { eof = true; } } in.close(); } } out.close(); Runtime.getRuntime().exec("notepad " + location); DecimalFormat round = new DecimalFormat("#.###"); html.println("<html><h1>******* Perfect SSRs *******</h1>"); html.println("<h4>Results for project: " + organisms[i] + "</h4><h4>Search Parameters --> Minimum SSR Length (bp): " + minlen + "</h4>"); html.println( "<table border=\"1\"><tr><td><b>motif</b></td><td><b>count</b></td><td><b>bp</b></td><td><b>A%</b></td><td><b>T%</b></td><td><b>C%</b></td><td><b>G%</b></td><td><b>Relative Frequency</b></td><td><b>Abundance</b></td><td><b>Relative Abundance</b></td></tr>"); stats.println("******* Perfect SSRs *******"); stats.println("Results for project: " + organisms[i] + "\n Search Parameters --> Minimum SSR Length (bp): " + minlen); stats.println( " ____________________________________________________________________________________________________________ "); stats.println( "| | | | | | | | Relative | | Relative |"); stats.println( "| motif | count | bp | A% | T% | C% | G% | Frequency | Abundance | Abundance |"); stats.println( "|=======|=======|============|=======|=======|=======|=======|===============|===============|===============|"); int totalcount = 0; long bpcount = 0; int Aperc = 0; int Tperc = 0; int Gperc = 0; int Cperc = 0; float relfreq = 0; float abfreq = 0; long seqcount = 0; if (mono.isSelected()) { totalcount += countmono.get(i); bpcount += countmonore.get(i); } if (di.isSelected()) { totalcount += countdi.get(i); bpcount += countdire.get(i) * 2; } if (tri.isSelected()) { totalcount += counttri.get(i); bpcount += counttrire.get(i) * 3; } if (tetra.isSelected()) { totalcount += counttetra.get(i); bpcount += counttetrare.get(i) * 4; } if (penta.isSelected()) { totalcount += countpenta.get(i); bpcount += countpentare.get(i) * 5; } if (hexa.isSelected()) { totalcount += counthexa.get(i); bpcount += counthexare.get(i) * 6; } try { Class.forName("com.mysql.jdbc.Driver"); } catch (ClassNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } Connection con = null; try { con = DriverManager.getConnection("jdbc:mysql://localhost:3306", "biouser", "thesis2012"); } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } Statement st = null; try { st = con.createStatement(); } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } st.executeUpdate("use lobid"); seqcount = 0; if (filetype.contains("organisms")) { ResultSet rs = st.executeQuery( "SELECT end FROM slices INNER JOIN organism WHERE slices.org_id=organism.org_id AND organism.name='" + organisms[i] + "'"); while (rs.next()) { seqcount += Long.parseLong(rs.getString(1)); } } else if (filetype.contains("local")) { BufferedReader in = new BufferedReader( new FileReader("local/" + organisms[i] + "/index.txt")); int count = countlines("local/" + organisms[i] + "/index.txt"); for (int c = 0; c < count; c++) { String temp = in.readLine(); BufferedReader tmp = new BufferedReader( new FileReader("local/" + organisms[i] + "/" + temp + ".txt")); boolean eof = false; while (!eof) { String s = tmp.readLine(); if (s != null) { seqcount += s.length(); } else { eof = true; } } tmp.close(); } } if (mono.isSelected()) { Aperc += Amono.get(i); Tperc += Tmono.get(i); Gperc += Gmono.get(i); Cperc += Cmono.get(i); //lt.writeInt(countmono);lt.writeInt(countmonore);lt.writeFloat((float)Amono*100/countmonore);lt.writeFloat((float)Tmono*100/countmonore);lt.writeFloat((float)Gmono*100/countmonore);lt.writeFloat((float)Cmono*100/countmonore);lt.writeFloat((float) countmono / totalcount);lt.writeFloat((float) countmonore / seqcount);lt.writeFloat((float) countmonore / bpcount); stats.printf("|mono |" + cell(Integer.toString(countmono.get(i)), 7) + "|" + cell(Integer.toString(1 * countmonore.get(i)), 12) + "|%s|%s|%s|%s|" + cell((float) countmono.get(i) / totalcount, 15) + "|" + cell((float) countmonore.get(i) / seqcount, 15) + "|" + cell((float) countmonore.get(i) / bpcount, 15) + "|\n", cell((float) (Amono.get(i) * 100) / (countmonore.get(i)), 7), cell((float) (Tmono.get(i) * 100) / (countmonore.get(i)), 7), cell((float) (Cmono.get(i) * 100) / (countmonore.get(i)), 7), cell((float) (Gmono.get(i) * 100) / (countmonore.get(i)), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>mono</b></td><td>" + countmono.get(i) + "</td><td>" + (1 * countmonore.get(i)) + "</td><td>" + round.format((float) (Amono.get(i) * 100) / (countmonore.get(i))) + "</td><td>" + round.format((float) (Tmono.get(i) * 100) / (countmonore.get(i))) + "</td><td>" + round.format((float) (Cmono.get(i) * 100) / (countmonore.get(i))) + "</td><td>" + round.format((float) (Gmono.get(i) * 100) / (countmonore.get(i))) + "</td><td>" + round.format((float) countmono.get(i) / totalcount) + "</td><td>" + round.format((float) countmonore.get(i) / seqcount) + "</td><td>" + round.format((float) countmonore.get(i) / bpcount) + "</td></tr>"); } if (di.isSelected()) { Aperc += Adi.get(i); Tperc += Tdi.get(i); Gperc += Gdi.get(i); Cperc += Cdi.get(i); //lt.writeInt(countdi);lt.writeInt(countdire*2);lt.writeFloat((float)Adi*100/countdire*2);lt.writeFloat((float)Tdi*100/countdire*2);lt.writeFloat((float)Gdi*100/countdire*2);lt.writeFloat((float)Cdi*100/countdire*2);lt.writeFloat((float) countdi / totalcount);lt.writeFloat((float) countdire*2 / seqcount);lt.writeFloat((float) countdire*2 / bpcount); stats.printf("|di |" + cell(Integer.toString(countdi.get(i)), 7) + "|" + cell(Integer.toString(countdire.get(i) * 2), 12) + "|%s|%s|%s|%s|" + cell((float) countdi.get(i) / totalcount, 15) + "|" + cell((float) countdire.get(i) * 2 / seqcount, 15) + "|" + cell((float) countdire.get(i) * 2 / bpcount, 15) + "|\n", cell((float) (Adi.get(i) * 100) / (countdire.get(i) * 2), 7), cell((float) (Tdi.get(i) * 100) / (countdire.get(i) * 2), 7), cell((float) (Cdi.get(i) * 100) / (countdire.get(i) * 2), 7), cell((float) (Gdi.get(i) * 100) / (countdire.get(i) * 2), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>di</b></td><td>" + countdi.get(i) + "</td><td>" + (2 * countdire.get(i)) + "</td><td>" + round.format((float) (Adi.get(i) * 100) / (2 * countdire.get(i))) + "</td><td>" + round.format((float) (Tdi.get(i) * 100) / (2 * countdire.get(i))) + "</td><td>" + round.format((float) (Cdi.get(i) * 100) / (2 * countdire.get(i))) + "</td><td>" + round.format((float) (Gdi.get(i) * 100) / (2 * countdire.get(i))) + "</td><td>" + round.format((float) countdi.get(i) / totalcount) + "</td><td>" + round.format((float) 2 * countdire.get(i) / seqcount) + "</td><td>" + round.format((float) 2 * countdire.get(i) / bpcount) + "</td></tr>"); } if (tri.isSelected()) { Aperc += Atri.get(i); Tperc += Ttri.get(i); Gperc += Gtri.get(i); Cperc += Ctri.get(i); //lt.writeInt(counttri);lt.writeInt(counttrire*3);lt.writeFloat((float)Atri*100/counttrire*3);lt.writeFloat((float)Ttri*100/counttrire*3);lt.writeFloat((float)Gtri*100/counttrire*3);lt.writeFloat((float)Ctri*100/counttrire*3);lt.writeFloat((float) counttri / totalcount);lt.writeFloat((float) counttrire*3 / seqcount);lt.writeFloat((float) counttrire*3 / bpcount); stats.printf("|tri |" + cell(Integer.toString(counttri.get(i)), 7) + "|" + cell(Integer.toString(counttrire.get(i) * 3), 12) + "|%s|%s|%s|%s|" + cell((float) counttri.get(i) / totalcount, 15) + "|" + cell((float) counttrire.get(i) * 3 / seqcount, 15) + "|" + cell((float) counttrire.get(i) * 3 / bpcount, 15) + "|\n", cell((float) (Atri.get(i) * 100) / (counttrire.get(i) * 3), 7), cell((float) (Ttri.get(i) * 100) / (counttrire.get(i) * 3), 7), cell((float) (Ctri.get(i) * 100) / (counttrire.get(i) * 3), 7), cell((float) (Gtri.get(i) * 100) / (counttrire.get(i) * 3), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>tri</b></td><td>" + counttri.get(i) + "</td><td>" + (3 * counttrire.get(i)) + "</td><td>" + round.format( (float) (Atri.get(i) * 100) / (3 * counttrire.get(i))) + "</td><td>" + round.format( (float) (Ttri.get(i) * 100) / (3 * counttrire.get(i))) + "</td><td>" + round.format( (float) (Ctri.get(i) * 100) / (3 * counttrire.get(i))) + "</td><td>" + round.format( (float) (Gtri.get(i) * 100) / (3 * counttrire.get(i))) + "</td><td>" + round.format((float) counttri.get(i) / totalcount) + "</td><td>" + round.format((float) 3 * counttrire.get(i) / seqcount) + "</td><td>" + round.format((float) 3 * counttrire.get(i) / bpcount) + "</td></tr>"); } if (tetra.isSelected()) { Aperc += Atetra.get(i); Tperc += Ttetra.get(i); Gperc += Gtetra.get(i); Cperc += Ctetra.get(i); //lt.writeInt(counttetra);lt.writeInt(counttetrare*4);lt.writeFloat((float)Atetra*100/counttetrare*4);lt.writeFloat((float)Ttetra*100/counttetrare*4);lt.writeFloat((float)Gtetra*100/counttetrare*4);lt.writeFloat((float)Ctetra*100/counttetrare*4);lt.writeFloat((float) counttetra / totalcount);lt.writeFloat((float) counttetrare*4 / seqcount);lt.writeFloat((float) counttetrare*4 / bpcount); stats.printf("|tetra |" + cell(Integer.toString(counttetra.get(i)), 7) + "|" + cell(Integer.toString(counttetrare.get(i) * 4), 12) + "|%s|%s|%s|%s|" + cell((float) counttetra.get(i) / totalcount, 15) + "|" + cell((float) counttetrare.get(i) * 4 / seqcount, 15) + "|" + cell((float) counttetrare.get(i) * 4 / bpcount, 15) + "|\n", cell((float) (Atetra.get(i) * 100) / (counttetrare.get(i) * 4), 7), cell((float) (Ttetra.get(i) * 100) / (counttetrare.get(i) * 4), 7), cell((float) (Ctetra.get(i) * 100) / (counttetrare.get(i) * 4), 7), cell((float) (Gtetra.get(i) * 100) / (counttetrare.get(i) * 4), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>tetra</b></td><td>" + counttetra.get(i) + "</td><td>" + (4 * counttetrare.get(i)) + "</td><td>" + round.format( (float) (Atetra.get(i) * 100) / (4 * counttetrare.get(i))) + "</td><td>" + round.format( (float) (Ttetra.get(i) * 100) / (4 * counttetrare.get(i))) + "</td><td>" + round.format( (float) (Ctetra.get(i) * 100) / (4 * counttetrare.get(i))) + "</td><td>" + round.format( (float) (Gtetra.get(i) * 100) / (4 * counttetrare.get(i))) + "</td><td>" + round.format((float) counttetra.get(i) / totalcount) + "</td><td>" + round.format((float) 4 * counttetrare.get(i) / seqcount) + "</td><td>" + round.format((float) 4 * counttetrare.get(i) / bpcount) + "</td></tr>"); } if (penta.isSelected()) { Aperc += Apenta.get(i); Tperc += Tpenta.get(i); Gperc += Gpenta.get(i); Cperc += Cpenta.get(i); //lt.writeInt(countpenta);lt.writeInt(countpentare*5);lt.writeFloat((float)Apenta*100/countpentare*5);lt.writeFloat((float)Tpenta*100/countpentare*5);lt.writeFloat((float)Gpenta*100/countpentare*5);lt.writeFloat((float)Cpenta*100/countpentare*5);lt.writeFloat((float) countpenta / totalcount);lt.writeFloat((float) countpentare*5 / seqcount);lt.writeFloat((float) countpentare*5 / bpcount); stats.printf("|penta |" + cell(Integer.toString(countpenta.get(i)), 7) + "|" + cell(Integer.toString(countpentare.get(i) * 5), 12) + "|%s|%s|%s|%s|" + cell((float) countpenta.get(i) / totalcount, 15) + "|" + cell((float) countpentare.get(i) * 5 / seqcount, 15) + "|" + cell((float) countpentare.get(i) * 5 / bpcount, 15) + "|\n", cell((float) (Apenta.get(i) * 100) / (countpentare.get(i) * 5), 7), cell((float) (Tpenta.get(i) * 100) / (countpentare.get(i) * 5), 7), cell((float) (Cpenta.get(i) * 100) / (countpentare.get(i) * 5), 7), cell((float) (Gpenta.get(i) * 100) / (countpentare.get(i) * 5), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>penta</b></td><td>" + countpenta.get(i) + "</td><td>" + (5 * countpentare.get(i)) + "</td><td>" + round.format( (float) (Apenta.get(i) * 100) / (5 * countpentare.get(i))) + "</td><td>" + round.format( (float) (Tpenta.get(i) * 100) / (5 * countpentare.get(i))) + "</td><td>" + round.format( (float) (Cpenta.get(i) * 100) / (5 * countpentare.get(i))) + "</td><td>" + round.format( (float) (Gpenta.get(i) * 100) / (5 * countpentare.get(i))) + "</td><td>" + round.format((float) countpenta.get(i) / totalcount) + "</td><td>" + round.format((float) 5 * countpentare.get(i) / seqcount) + "</td><td>" + round.format((float) 5 * countpentare.get(i) / bpcount) + "</td></tr>"); } if (hexa.isSelected()) { Aperc += Ahexa.get(i); Tperc += Thexa.get(i); Gperc += Ghexa.get(i); Cperc += Chexa.get(i); //lt.writeInt(counthexa);lt.writeInt(counthexare*6);lt.writeFloat((float)Ahexa*100/counthexare*6);lt.writeFloat((float)Thexa*100/counthexare*6);lt.writeFloat((float)Ghexa*100/counthexare*6);lt.writeFloat((float)Chexa*100/counthexare*6);lt.writeFloat((float) counthexa / totalcount);lt.writeFloat((float) counthexare*6 / seqcount);lt.writeFloat((float) counthexare*6 / bpcount); stats.printf("|hexa |" + cell(Integer.toString(counthexa.get(i)), 7) + "|" + cell(Integer.toString(counthexare.get(i) * 6), 12) + "|%s|%s|%s|%s|" + cell((float) counthexa.get(i) / totalcount, 15) + "|" + cell((float) counthexare.get(i) * 6 / seqcount, 15) + "|" + cell((float) counthexare.get(i) * 6 / bpcount, 15) + "|\n", cell((float) (Ahexa.get(i) * 100) / (counthexare.get(i) * 6), 7), cell((float) (Thexa.get(i) * 100) / (counthexare.get(i) * 6), 7), cell((float) (Chexa.get(i) * 100) / (counthexare.get(i) * 6), 7), cell((float) (Ghexa.get(i) * 100) / (counthexare.get(i) * 6), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>hexa</b></td><td>" + counthexa.get(i) + "</td><td>" + (6 * counthexare.get(i)) + "</td><td>" + round.format( (float) (Ahexa.get(i) * 100) / (6 * counthexare.get(i))) + "</td><td>" + round.format( (float) (Thexa.get(i) * 100) / (6 * counthexare.get(i))) + "</td><td>" + round.format( (float) (Chexa.get(i) * 100) / (6 * counthexare.get(i))) + "</td><td>" + round.format( (float) (Ghexa.get(i) * 100) / (6 * counthexare.get(i))) + "</td><td>" + round.format((float) counthexa.get(i) / totalcount) + "</td><td>" + round.format((float) 6 * counthexare.get(i) / seqcount) + "</td><td>" + round.format((float) 6 * counthexare.get(i) / bpcount) + "</td></tr>"); } if (mono.isSelected()) { relfreq += (float) countmono.get(i) / totalcount; abfreq += (float) countmonore.get(i) / bpcount; } if (di.isSelected()) { relfreq += (float) countdi.get(i) / totalcount; abfreq += (float) countdire.get(i) * 2 / bpcount; } if (tri.isSelected()) { relfreq += (float) counttri.get(i) / totalcount; abfreq += (float) counttrire.get(i) * 3 / bpcount; } if (tetra.isSelected()) { relfreq += (float) counttetra.get(i) / totalcount; abfreq += (float) counttetrare.get(i) * 4 / bpcount; } if (penta.isSelected()) { relfreq += (float) countpenta.get(i) / totalcount; abfreq += (float) countpentare.get(i) * 5 / bpcount; } if (hexa.isSelected()) { relfreq += (float) counthexa.get(i) / totalcount; abfreq += (float) counthexare.get(i) * 6 / bpcount; } Globals.A = Aperc; Globals.T = Tperc; Globals.G = Gperc; Globals.C = Cperc; lt.writeLong(seqcount); lt.writeInt(totalcount); lt.writeLong(bpcount); lt.writeInt(Aperc); lt.writeInt(Tperc); lt.writeInt(Gperc); lt.writeInt(Cperc); stats.println("|TOTAL |" + cell(Integer.toString(totalcount), 7) + "|" + cell(Long.toString(bpcount), 12) + "|" + cell((float) Aperc * 100 / bpcount, 7) + "|" + cell((float) Tperc * 100 / bpcount, 7) + "|" + cell((float) Cperc * 100 / bpcount, 7) + "|" + cell((float) Gperc * 100 / bpcount, 7) + "|" + cell(relfreq, 15) + "|" + cell((float) bpcount / seqcount, 15) + "|" + cell((float) abfreq, 15) + "|"); stats.println( "|_______|_______|____________|_______|_______|_______|_______|_______________|_______________|_______________|"); stats.println("Genome length (bp): " + seqcount); stats.println("Relative Frequency: Count of each motif type / total SSR count"); stats.println("Abundance: bp of each motif type / total sequence bp"); stats.println( "Relative Abundance: bp of each motif type / total microsatellites bp"); stats.println(); stats.println(); stats.close(); lt.close(); html.println("<tr><td><b>TOTAL</b></td><td>" + totalcount + "</td><td>" + bpcount + "</td><td>" + round.format((float) Aperc * 100 / bpcount) + "</td><td>" + round.format((float) Tperc * 100 / bpcount) + "</td><td>" + round.format((float) Cperc * 100 / bpcount) + "</td><td>" + round.format((float) Gperc * 100 / bpcount) + "</td><td>" + round.format((float) relfreq) + "</td><td>" + round.format((float) bpcount / seqcount) + "</td><td>" + round.format((float) abfreq) + "</td></tr></table></html>"); html.close(); // it = map.keySet().iterator(); for (String key : map.keySet()) { map.get(key).refresh(); } List<String> n1 = new ArrayList<String>(); List<String> n2 = new ArrayList<String>(); List<String> n3 = new ArrayList<String>(); List<String> n4 = new ArrayList<String>(); List<String> n5 = new ArrayList<String>(); List<String> n6 = new ArrayList<String>(); Iterator<String> it = map.keySet().iterator(); while (it.hasNext()) { String next = it.next(); int len = next.length(); if (len == 1) n1.add(next); else if (len == 2) n2.add(next); else if (len == 3) n3.add(next); else if (len == 4) n4.add(next); else if (len == 5) n5.add(next); else if (len == 6) n6.add(next); } Collections.sort(n1); Collections.sort(n2); Collections.sort(n3); Collections.sort(n4); Collections.sort(n5); Collections.sort(n6); boolean[] id1 = new boolean[n1.size()]; boolean[] id2 = new boolean[n2.size()]; boolean[] id3 = new boolean[n3.size()]; boolean[] id4 = new boolean[n4.size()]; boolean[] id5 = new boolean[n5.size()]; boolean[] id6 = new boolean[n6.size()]; motifhtml.println("<html><head><title>Motif Statistics</title></head><body>"); int stand = checkStandardize(); // stand=2; debug if (stand == 0) { motifstats.println("**** Not Standardized ****"); motifhtml.println("<h1>**** Not Standardized ****</h1>"); Arrays.fill(id1, true); Arrays.fill(id2, true); Arrays.fill(id3, true); Arrays.fill(id4, true); Arrays.fill(id5, true); Arrays.fill(id6, true); } else { if (stand == 1) { motifstats.println("**** Partially Standardized ****"); motifhtml.println("<h1>**** Partially Standardized ****</h1>"); matrix = new parser(1); } if (stand == 2) { motifstats.println("**** Fully Standardized ****"); motifhtml.println("<h1>**** Fully Standardized ****</h1>"); matrix = new parser(2); } Arrays.fill(id1, true); Arrays.fill(id2, true); Arrays.fill(id3, true); Arrays.fill(id4, true); Arrays.fill(id5, true); Arrays.fill(id6, true); for (int n = 0; n < n1.size(); n++) { int id = -2; id = matrix.SearchMap(1, n1.get(n)); if (id >= 0) { int found = matrix.checkFound(1, id); if (found >= 0) { map.get(n1.get(found)).merge(map.get(n1.get(n))); id1[n] = false; // gia clean twn listwn apo merged TODO * } if (found == -4) { matrix.makeFound(1, id, n); } } } for (int n = 0; n < n2.size(); n++) { int id = -2; id = matrix.SearchMap(2, n2.get(n)); if (id >= 0) { int found = matrix.checkFound(2, id); if (found >= 0) { map.get(n2.get(found)).merge(map.get(n2.get(n))); id2[n] = false; // gia clean twn listwn apo merged TODO * } else { matrix.makeFound(2, id, n); id2[n] = true; } } } for (int n = 0; n < n3.size(); n++) { int id = -2; id = matrix.SearchMap(3, n3.get(n)); if (id >= 0) { int found = matrix.checkFound(3, id); if (found >= 0) { map.get(n3.get(found)).merge(map.get(n3.get(n))); id3[n] = false; // gia clean twn listwn apo merged TODO * } else { matrix.makeFound(3, id, n); id3[n] = true; } } } for (int n = 0; n < n4.size(); n++) { int id = -2; id = matrix.SearchMap(4, n4.get(n)); if (id >= 0) { int found = matrix.checkFound(4, id); if (found >= 0) { map.get(n4.get(found)).merge(map.get(n4.get(n))); id4[n] = false; // gia clean twn listwn apo merged TODO * } else { matrix.makeFound(4, id, n); id4[n] = true; } } } for (int n = 0; n < n5.size(); n++) { int id = -2; id = matrix.SearchMap(5, n5.get(n)); if (id >= 0) { int found = matrix.checkFound(5, id); if (found >= 0) { map.get(n5.get(found)).merge(map.get(n5.get(n))); id5[n] = false; // gia clean twn listwn apo merged TODO * } else { matrix.makeFound(5, id, n); id5[n] = true; } } } for (int n = 0; n < n6.size(); n++) { int id = -2; id = matrix.SearchMap(6, n6.get(n)); if (id >= 0) { int found = matrix.checkFound(6, id); if (found >= 0) { map.get(n6.get(found)).merge(map.get(n6.get(n))); id6[n] = false; // gia clean twn listwn apo merged TODO * } else { matrix.makeFound(6, id, n); id6[n] = true; } } } } for (String key : map.keySet()) { map.get(key).refresh(); } motifstats.println( " Motif Count Repeats bp Avg_Length SD_Length Max_Length Avg_Repeats A% T% C% G% "); motifhtml.println( "<table border=\"1\"><tr><td><b>Motif</b></td><td><b>Count</b></td><td><b>Repeats</b></td><td><b>bp</b></td><td><b>Avg_Length</b></td><td><b>SD_Length</b></td><td><b>Max_Length</b></td><td><b>Avg_Repeats</b></td><td><b>A%</b></td><td><b>T%</b></td><td><b>C%</b></td><td><b>G%</b></td></tr>"); if (mono.isSelected()) { for (int z = 0; z < n1.size(); z++) { if (id1[z] && !map.get(n1.get(z)).getMotif().contains("N")) { motifstats.println(map.get(n1.get(z)).toString()); motifhtml.println(map.get(n1.get(z)).toHTML()); } } } if (di.isSelected()) { for (int z = 0; z < n2.size(); z++) { if (id2[z] && !map.get(n2.get(z)).getMotif().contains("N")) { motifstats.println(map.get(n2.get(z)).toString()); motifhtml.println(map.get(n2.get(z)).toHTML()); } } } if (tri.isSelected()) { for (int z = 0; z < n3.size(); z++) { if (id3[z] && !map.get(n3.get(z)).getMotif().contains("N")) { motifstats.println(map.get(n3.get(z)).toString()); motifhtml.println(map.get(n3.get(z)).toHTML()); } } } if (tetra.isSelected()) { for (int z = 0; z < n4.size(); z++) { if (id4[z] && !map.get(n4.get(z)).getMotif().contains("N")) { motifstats.println(map.get(n4.get(z)).toString()); motifhtml.println(map.get(n4.get(z)).toHTML()); } } } if (penta.isSelected()) { for (int z = 0; z < n5.size(); z++) { if (id5[z] && !map.get(n5.get(z)).getMotif().contains("N")) { motifstats.println(map.get(n5.get(z)).toString()); motifhtml.println(map.get(n5.get(z)).toHTML()); } } } if (hexa.isSelected()) { for (int z = 0; z < n6.size(); z++) { if (id6[z] && !map.get(n6.get(z)).getMotif().contains("N")) { motifstats.println(map.get(n6.get(z)).toString()); motifhtml.println(map.get(n6.get(z)).toHTML()); } } } motifstats.close(); motifhtml.println("</table></body></html>"); motifhtml.close(); Runtime.getRuntime().exec("notepad " + location2); } } catch (FileNotFoundException ex) { //msg.showMessageDialog(paneldown, "Update your selected species", "Error", JOptionPane.ERROR_MESSAGE); Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (ClassNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } else { msg.showMessageDialog(paneldown, "Minimum length requires an Integer", "Error", JOptionPane.ERROR_MESSAGE); } } else { msg.showMessageDialog(paneldown, "Please fill in the minimum length (Integers only)", "Error", JOptionPane.ERROR_MESSAGE); } } if (box2.isSelected()) { boolean gapisnumber = false; boolean minisnumber = false; int gap = 0; int min = 0; try { gap = Integer.parseInt(max.getText()); gapisnumber = true; } catch (NumberFormatException ex) { gapisnumber = false; } try { min = Integer.parseInt(minpregap.getText()); minisnumber = true; } catch (NumberFormatException ex) { minisnumber = false; } if (gapisnumber && minisnumber) { try { getImPerfectSSRs(organisms, min, flag, gap); for (int i = 0; i < organisms.length; i++) { PrintWriter stats = null; PrintWriter html = null; String location = ""; // 18/11/2013 added starting here String filetype = ""; String filepro = ""; if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } //List<String> files = getFiles(organisms[i], minlen, flag); // 18/11/2013 added ending here PrintWriter out = null; DataOutputStream lt = null; if (filetype.contains("organisms")) { File f = new File("organisms/" + organisms[i] + "/stats/"); if (!f.exists()) { f.mkdir(); } stats = new PrintWriter(new FileWriter( "organisms/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt", true)); lt = new DataOutputStream(new BufferedOutputStream( new FileOutputStream("organisms/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".imperf"))); html = new PrintWriter(new FileWriter( "organisms/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".html", true)); File fi = new File("organisms/" + organisms[i] + "/results/"); if (!fi.exists()) { fi.mkdir(); } String toopen = "organisms/" + organisms[i] + "/results/allImPerfect_" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; location = toopen; out = new PrintWriter(toopen); out.println("Results for organism: " + organisms[i] + "\t Search Parameters --> Maximum Mismatch length for ImPerfect SSRs : " + gap + " minimum SSR length before given gap: " + min); } else if (filetype.contains("local")) { File f = new File("local/" + organisms[i] + "/stats/"); if (!f.exists()) { f.mkdir(); } stats = new PrintWriter(new FileWriter( "local/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt", true)); lt = new DataOutputStream(new BufferedOutputStream(new FileOutputStream("local/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".imperf"))); html = new PrintWriter(new FileWriter( "local/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".html", true)); File fi = new File("local/" + organisms[i] + "/results/"); if (!fi.exists()) { fi.mkdir(); } String toopen = "local/" + organisms[i] + "/results/allImPerfect_" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; location = toopen; out = new PrintWriter(toopen); out.println("Results for project: " + organisms[i] + "\t Search Parameters --> Maximum Mismatch length for ImPerfect SSRs : " + gap + " minimum SSR length before given gap: " + min); } if (mono.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + min + "_" + gap + "_monoImPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int start = in.readInt(); int end = in.readInt(); if (!ssr.contains("N")) out.println("SSR: " + ssr + " start-end " + start + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); } catch (EOFException exc) { eof = true; } } in.close(); } } if (di.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + min + "_" + gap + "_diImPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int start = in.readInt(); int end = in.readInt(); if (!ssr.contains("N")) out.println("SSR: " + ssr + " start-end " + start + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); } catch (EOFException exc) { eof = true; } } in.close(); } } if (tri.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + min + "_" + gap + "_triImPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int start = in.readInt(); int end = in.readInt(); if (!ssr.contains("N")) out.println("SSR: " + ssr + " start-end " + start + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); } catch (EOFException exc) { eof = true; } } in.close(); } } if (tetra.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + min + "_" + gap + "_tetraImPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int start = in.readInt(); int end = in.readInt(); if (!ssr.contains("N")) out.println("SSR: " + ssr + " start-end " + start + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); } catch (EOFException exc) { eof = true; } } in.close(); } } if (penta.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + min + "_" + gap + "_pentaImPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int start = in.readInt(); int end = in.readInt(); if (!ssr.contains("N")) out.println("SSR: " + ssr + " start-end " + start + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); } catch (EOFException exc) { eof = true; } } in.close(); } } if (hexa.isSelected()) { // 18/11/2013 added starting here if (flag) { filetype = "organisms"; filepro = "organisms/" + organisms[i] + "/data/"; int ret = getOrganismStatus(organisms[i]); if (ret == -1) indexer = new Indexer(chromosomelist); else indexer = new Indexer(ret); } else { filetype = "local"; filepro = "local/" + organisms[i] + "/data/"; String indexfile = "local/" + organisms[i] + "/index.txt"; indexer = new Indexer(indexfile); } while (indexer.hasNext()) { String files = filepro + indexer.getNextFileName(); DataInputStream in = new DataInputStream( new BufferedInputStream(new FileInputStream( files + "_" + min + "_" + gap + "_hexaImPerfect.temp"))); boolean eof = false; while (!eof) { try { String ssr = in.readUTF(); int start = in.readInt(); int end = in.readInt(); if (!ssr.contains("N")) out.println("SSR: " + ssr + " start-end " + start + "-" + end + " Path(../data/chromosome): " + files.substring(0, files.lastIndexOf('.'))); } catch (EOFException exc) { eof = true; } } in.close(); } } out.close(); Runtime.getRuntime().exec("notepad " + location); DecimalFormat round = new DecimalFormat("#.###"); html.println("<html><h1>******* ImPerfect SSRs *******</h1>"); html.println("<h4>Results for project: " + organisms[i] + "</h4><h4>Search Parameters --> Maximum Mismatch length for ImPerfect SSRs (bp): " + gap + "</h4><h4>minimum SSR length before given Mismatch length (bp): " + min + "</h4>"); html.println( "<table border=\"1\"><tr><td><b>motif</b></td><td><b>count</b></td><td><b>bp</b></td><td><b>A%</b></td><td><b>T%</b></td><td><b>C%</b></td><td><b>G%</b></td><td><b>Relative Frequency</b></td><td><b>Abundance</b></td><td><b>Relative Abundance</b></td></tr>"); stats.println("******* ImPerfect SSRs *******"); stats.println("Results for project: " + organisms[i] + "\n Search Parameters --> Maximum Mismatch length for ImPerfect SSRs (bp): " + gap + " \nminimum SSR length before given Mismatch length (bp): " + min); stats.println( " ____________________________________________________________________________________________________________ "); stats.println( "| | | | | | | | Relative | | Relative |"); stats.println( "| motif | count | bp | A% | T% | C% | G% | Frequency | Abundance | Abundance |"); stats.println( "|=======|=======|============|=======|=======|=======|=======|===============|===============|===============|"); int totalcount = 0; long bpcount = 0; int Aperc = 0; int Tperc = 0; int Gperc = 0; int Cperc = 0; float relfreq = 0; float abfreq = 0; long seqcount = 0; if (mono.isSelected()) { totalcount += countmono.get(i); bpcount += countmonore.get(i); } if (di.isSelected()) { totalcount += countdi.get(i); bpcount += countdire.get(i); } if (tri.isSelected()) { totalcount += counttri.get(i); bpcount += counttrire.get(i); } if (tetra.isSelected()) { totalcount += counttetra.get(i); bpcount += counttetrare.get(i); } if (penta.isSelected()) { totalcount += countpenta.get(i); bpcount += countpentare.get(i); } if (hexa.isSelected()) { totalcount += counthexa.get(i); bpcount += counthexare.get(i); } try { Class.forName("com.mysql.jdbc.Driver"); } catch (ClassNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } Connection con = null; try { con = DriverManager.getConnection("jdbc:mysql://localhost:3306", "biouser", "thesis2012"); } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } Statement st = null; try { st = con.createStatement(); } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } st.executeUpdate("use lobid"); seqcount = 0; if (filetype.contains("organisms")) { ResultSet rs = st.executeQuery( "SELECT end FROM slices INNER JOIN organism WHERE slices.org_id=organism.org_id AND organism.name='" + organisms[i] + "'"); while (rs.next()) { seqcount += Long.parseLong(rs.getString(1)); } } else if (filetype.contains("local")) { BufferedReader in = new BufferedReader( new FileReader("local/" + organisms[i] + "/index.txt")); int count = countlines("local/" + organisms[i] + "/index.txt"); for (int c = 0; c < count; c++) { String temp = in.readLine(); BufferedReader tmp = new BufferedReader( new FileReader("local/" + organisms[i] + "/" + temp + ".txt")); boolean eof = false; while (!eof) { String s = tmp.readLine(); if (s != null) { seqcount += s.length(); } else { eof = true; } } tmp.close(); } } int tempmono = countmonore.get(i); int tempdi = countdire.get(i); int temptri = counttrire.get(i); int temptetra = counttetrare.get(i); int temppenta = countpentare.get(i); int temphexa = counthexare.get(i); if (tempmono == 0) tempmono = 1; if (tempdi == 0) tempdi = 1; if (temptri == 0) temptri = 1; if (temptetra == 0) temptetra = 1; if (temppenta == 0) temppenta = 1; if (temphexa == 0) temphexa = 1; if (mono.isSelected()) { Aperc += Amono.get(i); Tperc += Tmono.get(i); Gperc += Gmono.get(i); Cperc += Cmono.get(i); //lt.writeInt(countmono.get(i));lt.writeInt(countmonore.get(i));lt.writeFloat((float)Amono.get(i)*100/tempmono);lt.writeFloat((float)Tmono.get(i)*100/tempmono);lt.writeFloat((float)Gmono.get(i)*100/tempmono);lt.writeFloat((float)Cmono.get(i)*100/tempmono);lt.writeFloat((float) countmono.get(i) / totalcount);lt.writeFloat((float) countmonore.get(i) / seqcount);lt.writeFloat((float) countmonore.get(i) / bpcount); stats.printf("|mono |" + cell(Integer.toString(countmono.get(i)), 7) + "|" + cell(Integer.toString(countmonore.get(i)), 12) + "|%s|%s|%s|%s|" + cell((float) countmono.get(i) / totalcount, 15) + "|" + cell((float) countmonore.get(i) / seqcount, 15) + "|" + cell((float) countmonore.get(i) / bpcount, 15) + "|\n", cell((float) (Amono.get(i) * 100) / (tempmono), 7), cell((float) (Tmono.get(i) * 100) / (tempmono), 7), cell((float) (Cmono.get(i) * 100) / (tempmono), 7), cell((float) (Gmono.get(i) * 100) / (tempmono), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>mono</b></td><td>" + countmono.get(i) + "</td><td>" + (countmonore.get(i)) + "</td><td>" + round.format((float) (Amono.get(i) * 100) / (tempmono)) + "</td><td>" + round.format((float) (Tmono.get(i) * 100) / (tempmono)) + "</td><td>" + round.format((float) (Cmono.get(i) * 100) / (tempmono)) + "</td><td>" + round.format((float) (Gmono.get(i) * 100) / (tempmono)) + "</td><td>" + round.format((float) countmono.get(i) / totalcount) + "</td><td>" + round.format((float) countmonore.get(i) / seqcount) + "</td><td>" + round.format((float) countmonore.get(i) / bpcount) + "</td></tr>"); } if (di.isSelected()) { Aperc += Adi.get(i); Tperc += Tdi.get(i); Gperc += Gdi.get(i); Cperc += Cdi.get(i); //lt.writeInt(countdi.get(i));lt.writeInt(countdi.get(i)re.get(i));lt.writeFloat((float)Adi.get(i)*100/tempdi);lt.writeFloat((float)Tdi.get(i)*100/tempdi);lt.writeFloat((float)Gdi.get(i)*100/tempdi);lt.writeFloat((float)Cdi.get(i)*100/tempdi);lt.writeFloat((float) countdi.get(i) / totalcount);lt.writeFloat((float) countdi.get(i)re.get(i) / seqcount);lt.writeFloat((float) countdi.get(i)re.get(i) / bpcount); stats.printf( "|di |" + cell(Integer.toString(countdi.get(i)), 7) + "|" + cell(Integer.toString(countdire.get(i)), 12) + "|%s|%s|%s|%s|" + cell((float) countdi.get(i) / totalcount, 15) + "|" + cell((float) countdi.get(i) / seqcount, 15) + "|" + cell((float) countdi.get(i) / bpcount, 15) + "|\n", cell((float) (Adi.get(i) * 100) / (tempdi), 7), cell((float) (Tdi.get(i) * 100) / (tempdi), 7), cell((float) (Cdi.get(i) * 100) / (tempdi), 7), cell((float) (Gdi.get(i) * 100) / (tempdi), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>di</b></td><td>" + countdi.get(i) + "</td><td>" + (countdire.get(i)) + "</td><td>" + round.format((float) (Adi.get(i) * 100) / (tempdi)) + "</td><td>" + round.format((float) (Tdi.get(i) * 100) / (tempdi)) + "</td><td>" + round.format((float) (Cdi.get(i) * 100) / (tempdi)) + "</td><td>" + round.format((float) (Gdi.get(i) * 100) / (tempdi)) + "</td><td>" + round.format((float) countdi.get(i) / totalcount) + "</td><td>" + round.format((float) countdire.get(i) / seqcount) + "</td><td>" + round.format((float) countdire.get(i) / bpcount) + "</td></tr>"); } if (tri.isSelected()) { Aperc += Atri.get(i); Tperc += Ttri.get(i); Gperc += Gtri.get(i); Cperc += Ctri.get(i); //lt.writeInt(counttri.get(i));lt.writeInt(counttrire.get(i).get(i));lt.writeFloat((float)Atri.get(i)*100/temptri);lt.writeFloat((float)Ttri.get(i)*100/temptri);lt.writeFloat((float)Gtri.get(i)*100/temptri);lt.writeFloat((float)Ctri.get(i)*100/temptri);lt.writeFloat((float) counttri.get(i) / totalcount);lt.writeFloat((float) counttrire.get(i).get(i) / seqcount);lt.writeFloat((float) counttrire.get(i).get(i) / bpcount); stats.printf("|tri |" + cell(Integer.toString(counttri.get(i)), 7) + "|" + cell(Integer.toString(counttrire.get(i)), 12) + "|%s|%s|%s|%s|" + cell((float) counttri.get(i) / totalcount, 15) + "|" + cell((float) counttrire.get(i) / seqcount, 15) + "|" + cell((float) counttrire.get(i) / bpcount, 15) + "|\n", cell((float) (Atri.get(i) * 100) / (temptri), 7), cell((float) (Ttri.get(i) * 100) / (temptri), 7), cell((float) (Ctri.get(i) * 100) / (temptri), 7), cell((float) (Gtri.get(i) * 100) / (temptri), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>tri</b></td><td>" + counttri.get(i) + "</td><td>" + (counttrire.get(i)) + "</td><td>" + round.format((float) (Atri.get(i) * 100) / (temptri)) + "</td><td>" + round.format((float) (Ttri.get(i) * 100) / (temptri)) + "</td><td>" + round.format((float) (Ctri.get(i) * 100) / (temptri)) + "</td><td>" + round.format((float) (Gtri.get(i) * 100) / (temptri)) + "</td><td>" + round.format((float) counttri.get(i) / totalcount) + "</td><td>" + round.format((float) counttrire.get(i) / seqcount) + "</td><td>" + round.format((float) counttrire.get(i) / bpcount) + "</td></tr>"); } if (tetra.isSelected()) { Aperc += Atetra.get(i); Tperc += Ttetra.get(i); Gperc += Gtetra.get(i); Cperc += Ctetra.get(i); //lt.writeInt(counttetra.get(i));lt.writeInt(counttetrare.get(i));lt.writeFloat((float)Atetra.get(i)*100/temptetra);lt.writeFloat((float)Ttetra.get(i)*100/temptetra);lt.writeFloat((float)Gtetra.get(i)*100/temptetra);lt.writeFloat((float)Ctetra.get(i)*100/temptetra);lt.writeFloat((float) counttetra.get(i) / totalcount);lt.writeFloat((float) counttetrare.get(i) / seqcount);lt.writeFloat((float) counttetrare.get(i) / bpcount); stats.printf("|tetra |" + cell(Integer.toString(counttetra.get(i)), 7) + "|" + cell(Integer.toString(counttetrare.get(i)), 12) + "|%s|%s|%s|%s|" + cell((float) counttetra.get(i) / totalcount, 15) + "|" + cell((float) counttetrare.get(i) / seqcount, 15) + "|" + cell((float) counttetrare.get(i) / bpcount, 15) + "|\n", cell((float) (Atetra.get(i) * 100) / (temptetra), 7), cell((float) (Ttetra.get(i) * 100) / (temptetra), 7), cell((float) (Ctetra.get(i) * 100) / (temptetra), 7), cell((float) (Gtetra.get(i) * 100) / (temptetra), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>tetra</b></td><td>" + counttetra.get(i) + "</td><td>" + (counttetrare.get(i)) + "</td><td>" + round.format((float) (Atetra.get(i) * 100) / (temptetra)) + "</td><td>" + round.format((float) (Ttetra.get(i) * 100) / (temptetra)) + "</td><td>" + round.format((float) (Ctetra.get(i) * 100) / (temptetra)) + "</td><td>" + round.format((float) (Gtetra.get(i) * 100) / (temptetra)) + "</td><td>" + round.format((float) counttetra.get(i) / totalcount) + "</td><td>" + round.format((float) counttetrare.get(i) / seqcount) + "</td><td>" + round.format((float) counttetrare.get(i) / bpcount) + "</td></tr>"); } if (penta.isSelected()) { Aperc += Apenta.get(i); Tperc += Tpenta.get(i); Gperc += Gpenta.get(i); Cperc += Cpenta.get(i); //lt.writeInt(countpenta.get(i));lt.writeInt(countpentare.get(i));lt.writeFloat((float)Apenta.get(i)*100/temppenta);lt.writeFloat((float)Tpenta.get(i)*100/temppenta);lt.writeFloat((float)Gpenta.get(i)*100/temppenta);lt.writeFloat((float)Cpenta.get(i)*100/temppenta);lt.writeFloat((float) countpenta.get(i) / totalcount);lt.writeFloat((float) countpentare.get(i) / seqcount);lt.writeFloat((float) countpentare.get(i) / bpcount); stats.printf("|penta |" + cell(Integer.toString(countpenta.get(i)), 7) + "|" + cell(Integer.toString(countpentare.get(i)), 12) + "|%s|%s|%s|%s|" + cell((float) countpenta.get(i) / totalcount, 15) + "|" + cell((float) countpentare.get(i) / seqcount, 15) + "|" + cell((float) countpentare.get(i) / bpcount, 15) + "|\n", cell((float) (Apenta.get(i) * 100) / (temppenta), 7), cell((float) (Tpenta.get(i) * 100) / (temppenta), 7), cell((float) (Cpenta.get(i) * 100) / (temppenta), 7), cell((float) (Gpenta.get(i) * 100) / (temppenta), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>penta</b></td><td>" + countpenta.get(i) + "</td><td>" + (countpentare.get(i)) + "</td><td>" + round.format((float) (Apenta.get(i) * 100) / (temppenta)) + "</td><td>" + round.format((float) (Tpenta.get(i) * 100) / (temppenta)) + "</td><td>" + round.format((float) (Cpenta.get(i) * 100) / (temppenta)) + "</td><td>" + round.format((float) (Gpenta.get(i) * 100) / (temppenta)) + "</td><td>" + round.format((float) countpenta.get(i) / totalcount) + "</td><td>" + round.format((float) countpentare.get(i) / seqcount) + "</td><td>" + round.format((float) countpentare.get(i) / bpcount) + "</td></tr>"); } if (hexa.isSelected()) { Aperc += Ahexa.get(i); Tperc += Thexa.get(i); Gperc += Ghexa.get(i); Cperc += Chexa.get(i); //lt.writeInt(counthexa.get(i));lt.writeInt(counthexare.get(i));lt.writeFloat((float)Ahexa.get(i)*100/temphexa);lt.writeFloat((float)Thexa.get(i)*100/temphexa);lt.writeFloat((float)Ghexa.get(i)*100/temphexa);lt.writeFloat((float)Chexa.get(i)*100/temphexa);lt.writeFloat((float) counthexa.get(i) / totalcount);lt.writeFloat((float) counthexare.get(i) / seqcount);lt.writeFloat((float) counthexare.get(i) / bpcount); stats.printf("|hexa |" + cell(Integer.toString(counthexa.get(i)), 7) + "|" + cell(Integer.toString(counthexare.get(i)), 12) + "|%s|%s|%s|%s|" + cell((float) counthexa.get(i) / totalcount, 15) + "|" + cell((float) counthexare.get(i) / seqcount, 15) + "|" + cell((float) counthexare.get(i) / bpcount, 15) + "|\n", cell((float) (Ahexa.get(i) * 100) / (temphexa), 7), cell((float) (Thexa.get(i) * 100) / (temphexa), 7), cell((float) (Chexa.get(i) * 100) / (temphexa), 7), cell((float) (Ghexa.get(i) * 100) / (temphexa), 7)); stats.println( "|-------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>hexa</b></td><td>" + counthexa.get(i) + "</td><td>" + (counthexare.get(i)) + "</td><td>" + round.format((float) (Ahexa.get(i) * 100) / (temphexa)) + "</td><td>" + round.format((float) (Thexa.get(i) * 100) / (temphexa)) + "</td><td>" + round.format((float) (Chexa.get(i) * 100) / (temphexa)) + "</td><td>" + round.format((float) (Ghexa.get(i) * 100) / (temphexa)) + "</td><td>" + round.format((float) counthexa.get(i) / totalcount) + "</td><td>" + round.format((float) counthexare.get(i) / seqcount) + "</td><td>" + round.format((float) counthexare.get(i) / bpcount) + "</td></tr>"); } if (mono.isSelected()) { relfreq += (float) countmono.get(i) / totalcount; abfreq += (float) countmonore.get(i) / bpcount; } if (di.isSelected()) { relfreq += (float) countdi.get(i) / totalcount; abfreq += (float) countdire.get(i) / bpcount; } if (tri.isSelected()) { relfreq += (float) counttri.get(i) / totalcount; abfreq += (float) counttrire.get(i) / bpcount; } if (tetra.isSelected()) { relfreq += (float) counttetra.get(i) / totalcount; abfreq += (float) counttetrare.get(i) / bpcount; } if (penta.isSelected()) { relfreq += (float) countpenta.get(i) / totalcount; abfreq += (float) countpentare.get(i) / bpcount; } if (hexa.isSelected()) { relfreq += (float) counthexa.get(i) / totalcount; abfreq += (float) counthexare.get(i) / bpcount; } lt.writeLong(seqcount); lt.writeInt(totalcount); lt.writeLong(bpcount); lt.writeInt(Aperc); lt.writeInt(Tperc); lt.writeInt(Gperc); lt.writeInt(Cperc); stats.println("|TOTAL |" + cell(Integer.toString(totalcount), 7) + "|" + cell(Long.toString(bpcount), 12) + "|" + cell((float) Aperc * 100 / bpcount, 7) + "|" + cell((float) Tperc * 100 / bpcount, 7) + "|" + cell((float) Cperc * 100 / bpcount, 7) + "|" + cell((float) Gperc * 100 / bpcount, 7) + "|" + cell(relfreq, 15) + "|" + cell((float) bpcount / seqcount, 15) + "|" + cell((float) abfreq, 15) + "|"); stats.println( "|_______|_______|____________|_______|_______|_______|_______|_______________|_______________|_______________|"); stats.println("Genome length (bp): " + seqcount); stats.println("Relative Frequency: Count of each motif type / total SSR count"); stats.println("Abundance: bp of each motif type / total sequence bp"); stats.println( "Relative Abundance: bp of each motif type / total microsatellites bp"); stats.println(); stats.println(); stats.close(); lt.close(); html.println("<tr><td><b>TOTAL</b></td><td>" + totalcount + "</td><td>" + bpcount + "</td><td>" + round.format((float) Aperc * 100 / bpcount) + "</td><td>" + round.format((float) Tperc * 100 / bpcount) + "</td><td>" + round.format((float) Cperc * 100 / bpcount) + "</td><td>" + round.format((float) Gperc * 100 / bpcount) + "</td><td>" + round.format((float) relfreq) + "</td><td>" + round.format((float) bpcount / seqcount) + "</td><td>" + round.format((float) abfreq) + "</td></tr></table></html>"); html.close(); } } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (ClassNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); //msg.showMessageDialog(paneldown, "Update your selected species", "Error", JOptionPane.ERROR_MESSAGE); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } else { if (!gapisnumber && !minisnumber) { msg.showMessageDialog(paneldown, "Fill in Mismatch length for Imperfect SSRs \n and the minimum sequence length before the Mismatch length\n (Integers only)", "Error", JOptionPane.ERROR_MESSAGE); } else { if (!gapisnumber) { msg.showMessageDialog(paneldown, "Fill in Mismatch length for Imperfect SSRs (Integer only)", "Error", JOptionPane.ERROR_MESSAGE); } if (!minisnumber) { msg.showMessageDialog(paneldown, "Fill in the minimum sequence length before the Mismatch length (Integer only)", "Error", JOptionPane.ERROR_MESSAGE); } } } } if (box3.isSelected()) { boolean lenisnumber; boolean gapisnumber; int minlen = 0; int gap = 0; if (!com.isSelected() && !incom.isSelected()) { msg.showMessageDialog(paneldown, "Select a Compound SSR Option", "Error", JOptionPane.ERROR_MESSAGE); } else { try { minlen = Integer.parseInt(score.getText()); lenisnumber = true; } catch (NumberFormatException ex) { lenisnumber = false; } try { gap = Integer.parseInt(maxgapcomp.getText()); gapisnumber = true; } catch (NumberFormatException ex) { gapisnumber = false; } if (lenisnumber && gapisnumber) { if (com.isSelected()) { try { getCompoundPerfectSSRs(organisms, minlen, flag, gap); } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (ClassNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); //msg.showMessageDialog(paneldown, "Update your selected species", "Error", JOptionPane.ERROR_MESSAGE); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } if (incom.isSelected()) { try { getImPerfectCompoundSSRs(organisms, minlen, flag, gap); } catch (SQLException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (ClassNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); //msg.showMessageDialog(paneldown, "Update your selected species", "Error", JOptionPane.ERROR_MESSAGE); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } } else { if (!lenisnumber) { msg.showMessageDialog(paneldown, "Minimum length requires an Integer", "Error", JOptionPane.ERROR_MESSAGE); } if (!gapisnumber) { msg.showMessageDialog(paneldown, "Inter-repeat Region between compound SSRs requires an Integer", "Error", JOptionPane.ERROR_MESSAGE); } } } } if (box1.isSelected() || box2.isSelected() || box3.isSelected() || com.isSelected() || incom.isSelected()) { PrintWriter out = null; PrintWriter html = null; boolean orgs = true; DecimalFormat round = new DecimalFormat("#.###"); //String file=""; for (int i = 0; i < organisms.length; i++) { String file = "organisms/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; File stats = new File(file); if (!stats.exists()) { orgs = false; file = "local/" + organisms[i] + "/stats/" + "summary_statistics" + now.toString().replace(':', '_').replace(' ', '_') + ".txt"; } try { html = new PrintWriter( new FileWriter(file.substring(0, file.indexOf(".")) + ".html", true)); html.println("<html><h1>******* SUMMARY TABLE *******</h1>"); html.println( "<table border=\"1\"><tr><td>type</td><td><b>count</b></td><td><b>bp</b></td><td><b>A%</b></td><td><b>T%</b></td><td><b>C%</b></td><td><b>G%</b></td><td><b>Relative Frequency</b></td><td><b>Abundance</b></td><td><b>Relative Abundance</b></td></tr>"); out = new PrintWriter(new FileWriter(file, true)); out.println("******* SUMMARY TABLE *******"); out.println( " _________________________________________________________________________________________________________________ "); out.println( "| | | | | | | | Relative | | Relative |"); out.println( "| type | count | bp | A% | T% | C% | G% | Frequency | Abundance | Abundance |"); out.println( "|============|=======|============|=======|=======|=======|=======|===============|===============|===============|"); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } //TOTAL long seqcount = 0; int SSRcount = 0; int SSRbpcount = 0; int A = 0, T = 0, G = 0, C = 0; float relfreq = 0; float abfreq = 0; //perfect int pcount = 0; long pbpcount = 0; int Ap = 0, Tp = 0, Gp = 0, Cp = 0; //imperfect int imcount = 0; long imbpcount = 0; int Aim = 0, Tim = 0, Gim = 0, Cim = 0; //compound perfect int ccount = 0; long cbpcount = 0; int Ac = 0, Tc = 0, Gc = 0, Cc = 0; //compound imperfect int cicount = 0; long cibpcount = 0; int Aci = 0, Tci = 0, Gci = 0, Cci = 0; if (box1.isSelected()) { DataInputStream in = null; if (orgs) { try { in = new DataInputStream( new BufferedInputStream(new FileInputStream("organisms/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".perf"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } else { try { in = new DataInputStream(new BufferedInputStream(new FileInputStream("local/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".perf"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } try { seqcount = in.readLong(); pcount = in.readInt(); pbpcount = in.readLong(); Ap = in.readInt(); Tp = in.readInt(); Gp = in.readInt(); Cp = in.readInt(); A += Ap; T += Tp; G += Gp; C += Cp; SSRcount += pcount; SSRbpcount += pbpcount; in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } if (box2.isSelected()) { DataInputStream in = null; if (orgs) { try { in = new DataInputStream( new BufferedInputStream(new FileInputStream("organisms/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".imperf"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } else { try { in = new DataInputStream(new BufferedInputStream(new FileInputStream("local/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".imperf"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } try { seqcount = in.readLong(); imcount = in.readInt(); imbpcount = in.readLong(); Aim = in.readInt(); Tim = in.readInt(); Gim = in.readInt(); Cim = in.readInt(); A += Aim; T += Tim; G += Gim; C += Cim; SSRcount += imcount; SSRbpcount += imbpcount; in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } if (box3.isSelected()) { if (com.isSelected()) { DataInputStream in = null; if (orgs) { try { in = new DataInputStream(new BufferedInputStream( new FileInputStream("organisms/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".compp"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } else { try { in = new DataInputStream(new BufferedInputStream( new FileInputStream("local/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".compp"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } try { seqcount = in.readLong(); ccount = in.readInt(); cbpcount = in.readInt(); Ac = in.readInt(); Tc = in.readInt(); Gc = in.readInt(); Cc = in.readInt(); A += Ac; T += Tc; G += Gc; C += Cc; SSRcount += ccount; SSRbpcount += cbpcount; in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } if (incom.isSelected()) { DataInputStream in = null; if (orgs) { try { in = new DataInputStream(new BufferedInputStream( new FileInputStream("organisms/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".compim"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } else { try { in = new DataInputStream(new BufferedInputStream( new FileInputStream("local/" + organisms[i] + "/data/" + now.toString().replace(':', '_').replace(' ', '_') + ".compim"))); } catch (FileNotFoundException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } try { seqcount = in.readLong(); cicount = in.readInt(); cibpcount = in.readInt(); Aci = in.readInt(); Tci = in.readInt(); Gci = in.readInt(); Cci = in.readInt(); A += Aci; T += Tci; G += Gci; C += Cci; SSRcount += cicount; SSRbpcount += cibpcount; in.close(); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } } if (box1.isSelected()) { relfreq += (float) pcount / SSRcount; abfreq += (float) pbpcount / SSRbpcount; out.printf( "|Perfect |" + cell(Integer.toString(pcount), 7) + "|" + cell(Long.toString(pbpcount), 12) + "|%s|%s|%s|%s|" + cell((float) pcount / SSRcount, 15) + "|" + cell((float) pbpcount / seqcount, 15) + "|" + cell((float) pbpcount / SSRbpcount, 15) + "|\n", cell((float) (Ap * 100) / (SSRbpcount), 7), cell((float) (Tp * 100) / (SSRbpcount), 7), cell((float) (Cp * 100) / (SSRbpcount), 7), cell((float) (Gp * 100) / (SSRbpcount), 7)); out.println( "|------------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>Perfect</b></td><td>" + pcount + "</td><td>" + pbpcount + "</td><td>" + round.format((float) Ap * 100 / SSRbpcount) + "</td><td>" + round.format((float) Tp * 100 / SSRbpcount) + "</td><td>" + round.format((float) Cp * 100 / SSRbpcount) + "</td><td>" + round.format((float) Gp * 100 / SSRbpcount) + "</td><td>" + round.format((float) pcount / SSRcount) + "</td><td>" + round.format((float) pbpcount / seqcount) + "</td><td>" + round.format((float) pbpcount / SSRbpcount) + "</td></tr>"); } if (box2.isSelected()) { relfreq += (float) imcount / SSRcount; abfreq += (float) imbpcount / SSRbpcount; out.printf( "|Imperfect |" + cell(Integer.toString(imcount), 7) + "|" + cell(Long.toString(imbpcount), 12) + "|%s|%s|%s|%s|" + cell((float) imcount / SSRcount, 15) + "|" + cell((float) imbpcount / seqcount, 15) + "|" + cell((float) imbpcount / SSRbpcount, 15) + "|\n", cell((float) (Aim * 100) / (SSRbpcount), 7), cell((float) (Tim * 100) / (SSRbpcount), 7), cell((float) (Cim * 100) / (SSRbpcount), 7), cell((float) (Gim * 100) / (SSRbpcount), 7)); out.println( "|------------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>Imperfect</b></td><td>" + imcount + "</td><td>" + imbpcount + "</td><td>" + round.format((float) Aim * 100 / SSRbpcount) + "</td><td>" + round.format((float) Tim * 100 / SSRbpcount) + "</td><td>" + round.format((float) Cim * 100 / SSRbpcount) + "</td><td>" + round.format((float) Gim * 100 / SSRbpcount) + "</td><td>" + round.format((float) imcount / SSRcount) + "</td><td>" + round.format((float) imbpcount / seqcount) + "</td><td>" + round.format((float) imbpcount / SSRbpcount) + "</td></tr>"); } if (box3.isSelected()) { if (com.isSelected()) { abfreq += (float) cbpcount / SSRbpcount; relfreq += (float) ccount / SSRcount; out.printf( "|Compound Per|" + cell(Integer.toString(ccount), 7) + "|" + cell(Long.toString(cbpcount), 12) + "|%s|%s|%s|%s|" + cell((float) ccount / SSRcount, 15) + "|" + cell((float) cbpcount / seqcount, 15) + "|" + cell((float) cbpcount / SSRbpcount, 15) + "|\n", cell((float) (Ac * 100) / (SSRbpcount), 7), cell((float) (Tc * 100) / (SSRbpcount), 7), cell((float) (Cc * 100) / (SSRbpcount), 7), cell((float) (Gc * 100) / (SSRbpcount), 7)); out.println( "|------------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>Compound Perf.</b></td><td>" + ccount + "</td><td>" + cbpcount + "</td><td>" + round.format((float) Ac * 100 / SSRbpcount) + "</td><td>" + round.format((float) Tc * 100 / SSRbpcount) + "</td><td>" + round.format((float) Cc * 100 / SSRbpcount) + "</td><td>" + round.format((float) Gc * 100 / SSRbpcount) + "</td><td>" + round.format((float) ccount / SSRcount) + "</td><td>" + round.format((float) cbpcount / seqcount) + "</td><td>" + round.format((float) cbpcount / SSRbpcount) + "</td></tr>"); } if (incom.isSelected()) { abfreq += (float) cibpcount / SSRbpcount; relfreq += (float) cicount / SSRcount; out.printf( "|Compound Imp|" + cell(Integer.toString(cicount), 7) + "|" + cell(Long.toString(cibpcount), 12) + "|%s|%s|%s|%s|" + cell((float) cicount / SSRcount, 15) + "|" + cell((float) cibpcount / seqcount, 15) + "|" + cell((float) cibpcount / SSRbpcount, 15) + "|\n", cell((float) (Aci * 100) / (SSRbpcount), 7), cell((float) (Tci * 100) / (SSRbpcount), 7), cell((float) (Cci * 100) / (SSRbpcount), 7), cell((float) (Gci * 100) / (SSRbpcount), 7)); out.println( "|------------|-------|------------|-------|-------|-------|-------|---------------|---------------|---------------|"); html.println("<tr><td><b>Compound Imperf.</b></td><td>" + cicount + "</td><td>" + cibpcount + "</td><td>" + round.format((float) Aci * 100 / SSRbpcount) + "</td><td>" + round.format((float) Tci * 100 / SSRbpcount) + "</td><td>" + round.format((float) Cci * 100 / SSRbpcount) + "</td><td>" + round.format((float) Gci * 100 / SSRbpcount) + "</td><td>" + round.format((float) cicount / SSRcount) + "</td><td>" + round.format((float) cibpcount / seqcount) + "</td><td>" + round.format((float) cibpcount / SSRbpcount) + "</td></tr>"); } } out.println("|TOTAL |" + cell(Integer.toString(SSRcount), 7) + "|" + cell(Long.toString(SSRbpcount), 12) + "|" + cell((float) A * 100 / SSRbpcount, 7) + "|" + cell((float) T * 100 / SSRbpcount, 7) + "|" + cell((float) C * 100 / SSRbpcount, 7) + "|" + cell((float) G * 100 / SSRbpcount, 7) + "|" + cell(relfreq, 15) + "|" + cell((float) SSRbpcount / seqcount, 15) + "|" + cell((float) abfreq, 15) + "|"); out.println( "|____________|_______|____________|_______|_______|_______|_______|_______________|_______________|_______________|"); out.println("Genome length (bp): " + seqcount); out.println("Relative Frequency: Count of each motif type / total SSR count"); out.println("Abundance: bp of each motif type / total sequence bp"); out.println("Relative Abundance: bp of each motif type / total microsatellites bp"); out.println(); out.println(); out.close(); html.println("<tr><td><b>TOTAL</b></td><td>" + SSRcount + "</td><td>" + SSRbpcount + "</td><td>" + round.format((float) A * 100 / SSRbpcount) + "</td><td>" + round.format((float) T * 100 / SSRbpcount) + "</td><td>" + round.format((float) C * 100 / SSRbpcount) + "</td><td>" + round.format((float) G * 100 / SSRbpcount) + "</td><td>" + round.format((float) relfreq) + "</td><td>" + round.format((float) SSRbpcount / seqcount) + "</td><td>" + round.format((float) abfreq) + "</td></tr></table></html>"); html.close(); try { Runtime.getRuntime().exec("notepad " + file); } catch (IOException ex) { Logger.getLogger(StatsSelection.class.getName()).log(Level.SEVERE, null, ex); } } } setCursor(Cursor.getPredefinedCursor(Cursor.DEFAULT_CURSOR)); } }); selectsp = new JButton("Select new Species"); selectsp.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { try { input_frame frame = new input_frame(); } catch (ClassNotFoundException ex) { Logger.getLogger(updateframe.class.getName()).log(Level.SEVERE, null, ex); } catch (SQLException ex) { Logger.getLogger(updateframe.class.getName()).log(Level.SEVERE, null, ex); } dispose(); } }); quit = new JButton("Quit"); quit.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent e) { System.exit(0); } }); tab = new JTabbedPane(); tab.setSize(790, 590); // about us panel ImageIcon image = new ImageIcon("miga.png"); JLabel label = new JLabel("", image, JLabel.CENTER); contact = new JLabel( "<html><b><p>Machine Learning and Knowledge Discovery Group</p><p>Computer Science Department</p><p>Aristotle University of Thessaloniki</p><p> </p><p>In collaboration with the laboratory of</p><p> </p><p>Population Genetics of Animal Organisms</p><p>Department of Genetics, Development and Molecular Biology</p><p>School of Biology</p><p>Aristotle University of Thessaloniki</p><p> </p><p> </p><p>For any comments or information please contact with:</p><p><a href=\"mailto:ikavak@csd.auth.gr\">ikavak@csd.auth.gr</a></p></b></html>"); about = new JPanel(); about.setLayout(new GridBagLayout()); GridBagConstraints ab = new GridBagConstraints(); ab.fill = GridBagConstraints.WEST; ab.weightx = 0.5; ab.weighty = 0.5; ab.gridx = 0; ab.gridy = 0; about.add(label, ab); ab.fill = GridBagConstraints.WEST; ab.weightx = 0.5; ab.weighty = 0.5; ab.gridx = 0; ab.gridy = 1; about.add(contact, ab); //end of about us //below are the rest of th panels paneltop = new JPanel(); TitledBorder t = BorderFactory.createTitledBorder("Select Period"); paneltop.setBorder(t); paneltop.setLayout(new GridBagLayout()); GridBagConstraints pt = new GridBagConstraints(); pt.fill = GridBagConstraints.HORIZONTAL; pt.weightx = 0.5; pt.weighty = 0.5; pt.gridx = 0; pt.gridy = 0; paneltop.add(mono, pt); pt.fill = GridBagConstraints.HORIZONTAL; pt.weightx = 0.5; pt.weighty = 0.5; pt.gridx = 0; pt.gridy = -1; paneltop.add(di, pt); pt.fill = GridBagConstraints.HORIZONTAL; pt.weightx = 0.5; pt.weighty = 0.5; pt.gridx = 0; pt.gridy = -2; paneltop.add(tri, pt); pt.fill = GridBagConstraints.HORIZONTAL; pt.weightx = 0.5; pt.weighty = 0.5; pt.gridx = 0; pt.gridy = -3; paneltop.add(tetra, pt); pt.fill = GridBagConstraints.HORIZONTAL; pt.weightx = 0.5; pt.weighty = 0.5; pt.gridx = 0; pt.gridy = -4; paneltop.add(penta, pt); pt.fill = GridBagConstraints.HORIZONTAL; pt.weightx = 0.5; pt.weighty = 0.5; pt.gridx = 0; pt.gridy = -5; paneltop.add(hexa, pt); panel1 = new JPanel(); TitledBorder title = BorderFactory.createTitledBorder("Type"); panel1.setBorder(title); panel1.setLayout(new GridBagLayout()); GridBagConstraints a = new GridBagConstraints(); a.fill = GridBagConstraints.HORIZONTAL; a.weightx = 0.5; a.weighty = 0.5; a.gridx = 0; a.gridy = 0; panel1.add(select, a); a.fill = GridBagConstraints.HORIZONTAL; a.weightx = 0.5; a.weighty = 0.5; a.gridx = 0; a.gridy = -1; panel1.add(box1, a); a.fill = GridBagConstraints.HORIZONTAL; a.weightx = 0.5; a.weighty = 0.5; a.gridx = 0; a.gridy = -2; panel1.add(box2, a); a.fill = GridBagConstraints.HORIZONTAL; a.weightx = 0.5; a.weighty = 0.5; a.gridx = 0; a.gridy = -3; panel1.add(box3, a); panel2 = new JPanel(); panel2.setVisible(false); TitledBorder title2 = BorderFactory.createTitledBorder("More Options"); panel2.setBorder(title2); panel2.setLayout(new GridBagLayout()); GridBagConstraints b = new GridBagConstraints(); b.fill = GridBagConstraints.HORIZONTAL; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 0; b.gridy = 0; panel2.add(minimumssrlen, b); b.fill = GridBagConstraints.CENTER; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 1; b.gridy = 0; panel2.add(score, b); b.fill = GridBagConstraints.HORIZONTAL; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 0; b.gridy = -1; panel2.add(gapmax, b); b.fill = GridBagConstraints.CENTER; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 1; b.gridy = -1; panel2.add(max, b); b.fill = GridBagConstraints.HORIZONTAL; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 0; b.gridy = -2; panel2.add(minlenpregap, b); b.fill = GridBagConstraints.CENTER; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 1; b.gridy = -2; panel2.add(minpregap, b); b.fill = GridBagConstraints.HORIZONTAL; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 0; b.gridy = -3; panel2.add(gapcomp, b); b.fill = GridBagConstraints.CENTER; b.weightx = 0.5; b.weighty = 0.5; b.gridx = 1; b.gridy = -3; panel2.add(maxgapcomp, b); paneldownleft = new JPanel(); paneldownleft.setVisible(false); TitledBorder titledl = BorderFactory.createTitledBorder("Compound SSR options"); paneldownleft.setBorder(titledl); paneldownleft.setLayout(new GridBagLayout()); GridBagConstraints dl = new GridBagConstraints(); dl.fill = GridBagConstraints.CENTER; dl.weightx = 0.5; dl.weighty = 0.5; dl.gridx = 0; dl.gridy = 0; paneldownleft.add(com, dl); dl.fill = GridBagConstraints.CENTER; dl.weightx = 0.5; dl.weighty = 0.5; dl.gridx = 0; dl.gridy = -1; paneldownleft.add(incom, dl); paneldownright = new JPanel(); paneldownright.setLayout(new GridBagLayout()); GridBagConstraints dr = new GridBagConstraints(); dr.fill = GridBagConstraints.CENTER; dr.weightx = 0.5; dr.weighty = 0.5; dr.gridx = 0; dr.gridy = 0; paneldownright.add(show, dr); dr.fill = GridBagConstraints.CENTER; dr.weightx = 0.5; dr.weighty = 0.5; dr.gridx = 0; dr.gridy = -1; paneldownright.add(selectsp, dr); dr.fill = GridBagConstraints.CENTER; dr.weightx = 0.5; dr.weighty = 0.5; dr.gridx = 0; dr.gridy = -2; paneldownright.add(quit, dr); panelup = new JPanel(); TitledBorder titleup = BorderFactory.createTitledBorder("Statistics"); panelup.setBorder(titleup); panelup.setLayout(new GridBagLayout()); GridBagConstraints c = new GridBagConstraints(); c.fill = GridBagConstraints.HORIZONTAL; c.weightx = 0.5; c.weighty = 0.5; c.gridx = 0; c.gridy = 0; panelup.add(paneltop, c); c.fill = GridBagConstraints.HORIZONTAL; c.weightx = 0.5; c.weighty = 0.5; c.gridx = 1; c.gridy = 0; panelup.add(panel1, c); c.fill = GridBagConstraints.HORIZONTAL; c.weightx = 0.5; c.weighty = 0.5; c.gridx = 0; c.gridy = -1; panelup.add(paneldownleft, c); c.fill = GridBagConstraints.HORIZONTAL; c.weightx = 0.5; c.weighty = 0.5; c.gridx = 1; c.gridy = -1; panelup.add(panel2, c); c.fill = GridBagConstraints.HORIZONTAL; c.weightx = 0.5; c.weighty = 0.5; c.gridx = 0; c.gridy = -2; panelup.add(paneldownright, c); TitledBorder s = BorderFactory.createTitledBorder("Standardization"); std.setBorder(s); std.add(no_st); std.add(part_st); std.add(full_st); std.setEnabled(false); c.fill = GridBagConstraints.HORIZONTAL; c.weightx = 0.5; c.weighty = 0.5; c.gridx = 0; c.gridy = -3; panelup.add(std, c); tab.add(panelup, "Statistics"); panelshow = new JPanel(); TitledBorder ps = BorderFactory.createTitledBorder("Sequence Retrieval"); panelshow.setBorder(ps); panelshow.setLayout(new GridBagLayout()); GridBagConstraints psg = new GridBagConstraints(); panelshowup = new JPanel(); panelshowup.setLayout(new GridBagLayout()); GridBagConstraints p = new GridBagConstraints(); p.fill = GridBagConstraints.WEST; p.weightx = 0.5; p.weighty = 0.5; p.gridx = 0; p.gridy = 0; panelshowup.add(lab, p); panelshowd = new JPanel(); panelshowd.setLayout(new GridBagLayout()); GridBagConstraints pd = new GridBagConstraints(); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 0; pd.gridy = -1; panelshowd.add(startlab, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 1; pd.gridy = -1; panelshowd.add(startnum, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 0; pd.gridy = -2; panelshowd.add(endlab, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 1; pd.gridy = -2; panelshowd.add(endnum, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 0; pd.gridy = -3; panelshowd.add(titlelab, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 1; pd.gridy = -3; panelshowd.add(titlef, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 0; pd.gridy = -4; panelshowd.add(flk, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 1; pd.gridy = -4; panelshowd.add(new JLabel(" "), pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 0; pd.gridy = -5; panelshowd.add(flankst, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 1; pd.gridy = -5; panelshowd.add(flankstn, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 0; pd.gridy = -6; panelshowd.add(flankend, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 1; pd.gridy = -6; panelshowd.add(flankendn, pd); pd.fill = GridBagConstraints.CENTER; pd.weightx = 0.5; pd.weighty = 0.5; pd.gridx = 1; pd.gridy = -7; panelshowd.add(retrieve, pd); psg.fill = GridBagConstraints.CENTER; psg.weightx = 0.5; psg.weighty = 0.5; psg.gridx = 0; psg.gridy = 0; panelshow.add(panelshowup, psg); psg.fill = GridBagConstraints.CENTER; psg.weightx = 0.5; psg.weighty = 0.5; psg.gridx = 0; psg.gridy = -1; panelshow.add(panelshowd, psg); psg.fill = GridBagConstraints.CENTER; psg.weightx = 0.5; psg.weighty = 0.5; psg.gridx = 0; psg.gridy = -2; panelshow.add(sbrText, psg); tab.add(panelshow, "Sequence Retrieval"); tab.add(about, "About us"); add(tab); setVisible(true); }