To specify the start and terminal points in Well-Known Text format, a simple SELECT query will suffice:
mysql>
mysql>
mysql> SET @ls = GEOMFROMTEXT('LINESTRING(2 2, 9 0, 9 9)');
Query OK, 0 rows affected (0.00 sec)
mysql>
mysql> SELECT ASTEXT(STARTPOINT(@ls)), ASTEXT(ENDPOINT(@ls));
+-------------------------+-----------------------+
| ASTEXT(STARTPOINT(@ls)) | ASTEXT(ENDPOINT(@ls)) |
+-------------------------+-----------------------+
| POINT(2 2) | POINT(9 9) |
+-------------------------+-----------------------+
1 row in set (0.00 sec)
mysql>
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